Park Sang-Ik, Jeong Cheol, Kim Ha-Hyun, Park Sung-Hee, Park Su-Jin, Hyun Bang-Hun, Yang Dong-Kun, Kim Sang-Ki, Kang Mun-Il, Cho Kyoung-Oh
Bio-therapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Vet Microbiol. 2007 Sep 20;124(1-2):125-33. doi: 10.1016/j.vetmic.2007.03.010. Epub 2007 Mar 28.
Since the prevalence of bovine norovirus (BNoV) and their genetic diversity have only been reported in the USA, England, Germany and The Netherlands, this study examined the prevalence and genetic diversity of BNoVs in diarrheic calves in South Korea using 645 diarrheic fecal specimens from calves by RT-PCR and nested PCR assays. Overall, 9.3% of the diarrheic fecal samples tested positive for BNoVs by either RT-PCR or nested PCR, of which 5.9% samples also tested positive for other enteric pathogens including the bovine coronavirus, bovine viral diarrhea virus, bovine torovirus, bovine groups A, B and C rotaviruses, bovine enteric Nebraska-like calicivirus and Escherichia coli. The genetic diversity was determined by direct sequencing of the partial RdRp region of 12 BNoVs detected from the fecal samples by nested PCR. Among the BNoVs examined, one Korean BNoV strain had the highest nucleotide (86.8%) and amino acid (99.1%) identity with the genotype 1 BNoV (GIII-1) strain, while the remaining 11 Korean BNoVs shared a higher nucleotide (88.0-90.5%) and amino acid (93.5-99.1%) identity with the genotype 2 BNoV (GIII-2) strains. The phylogenetic data for the nucleotide and amino acid sequences also demonstrated that one Korean BNoV strain clustered with GIII-1 but the remaining eleven strains clustered with GIII-2. In conclusion, BNoV infections are endemic and there are two distinct genotypes with GIII-2 being the main genotype circulating in the calf population in South Korea.
由于仅在美国、英国、德国和荷兰报道过牛诺如病毒（BNoV）的流行情况及其遗传多样性，本研究采用逆转录聚合酶链反应（RT-PCR）和巢式PCR检测方法，对来自韩国腹泻犊牛的645份腹泻粪便样本进行检测，以研究BNoV在韩国腹泻犊牛中的流行情况及其遗传多样性。总体而言，通过RT-PCR或巢式PCR检测，9.3%的腹泻粪便样本BNoV呈阳性，其中5.9%的样本还对其他肠道病原体呈阳性，这些病原体包括牛冠状病毒、牛病毒性腹泻病毒、牛环曲病毒、牛A、B和C组轮状病毒、牛肠道内布拉斯加样杯状病毒和大肠杆菌。通过对巢式PCR从粪便样本中检测出的12株BNoV的部分RNA依赖的RNA聚合酶（RdRp）区域进行直接测序来确定遗传多样性。在所检测的BNoV中，一株韩国BNoV毒株与1型BNoV（GIII-1）毒株具有最高的核苷酸（86.8%）和氨基酸（99.1%）同一性，而其余11株韩国BNoV与2型BNoV（GIII-2）毒株具有更高的核苷酸（88.0 - 90.5%）和氨基酸（93.5 - 99.1%）同一性。核苷酸和氨基酸序列的系统发育数据也表明，一株韩国BNoV毒株与GIII-1聚类，但其余11株与GIII-2聚类。总之，BNoV感染呈地方性流行，有两种不同的基因型，其中GIII-2是在韩国犊牛群体中传播的主要基因型。
