土耳其牛诺如病毒系统发育的首次报告。
First report on the phylogeny of bovine norovirus in Turkey.
机构信息
Department of Virology, University of Istanbul, Avcilar, Turkey.
出版信息
Arch Virol. 2011 Jan;156(1):143-7. doi: 10.1007/s00705-010-0833-7. Epub 2010 Oct 22.
Bovine norovirus (BoNoV) is an important cause of diarrhea in calves and has been reported in several countries. The aims of this study were to investigate for the first time the presence of norovirus in Turkish calves by real-time reverse transcription-polymerase chain reaction (qRT-PCR) and to determine the phylogeny of any circulating strains. Fecal samples from 70 diarrheic calves were collected and analysed by SYBR Green qRT-PCR. BoNoV was detected in fecal samples from six calves. The capsid gene was partially sequenced, and phylogenetic analysis was performed. This showed that the six Turkish BoNoVs clustered with the GIII-2 prototype.
牛诺如病毒（BoNoV）是引起犊牛腹泻的重要原因，在多个国家均有报道。本研究首次通过实时逆转录聚合酶链反应（qRT-PCR）调查了土耳其犊牛中诺如病毒的存在情况，并确定了流行毒株的系统发育关系。采集了 70 份腹泻犊牛的粪便样本，并通过 SYBR Green qRT-PCR 进行分析。从 6 只犊牛的粪便样本中检测到了 BoNoV。对衣壳基因进行了部分测序，并进行了系统发育分析。结果表明，6 株土耳其 BoNoV 与 GIII-2 原型株聚为一簇。
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