Raulino-Domanski Fernanda, Potrich Michele, Freitas Patrícia F, Abdalla Fábio C, Martins Elias N, Lourenco Daniela A L, Costa-Maia Fabiana Martins
Animal Science Department, Federal Technological University of Paraná - UTFPR, Estrada para Boa Esperança, Dois Vizinhos, PR, Brazil.
Biological Sciences Department, Laboratory of Biological Control II, Federal Technological University of Paraná - UTFPR, Dois Vizinhos, PR, Brazil.
J Insect Sci. 2019 Mar 1;19(2). doi: 10.1093/jisesa/iez013.
Techniques for counting ovariole number in virgin and mated Apis mellifera L. queens have been described in previous studies. Having a systematic and fast way to collect this measurement can help accelerate bee breeding programs, because selection decisions can be taken faster. The aim of this work was to develop an efficient histological method to preserve ovaries that allows assessing the number of ovarioles in newly emerged virgin queens, and also in mated queens, in a shorter time than the methods already published. The proposed method resulted in images suitable for ovariole counting in both newly emerged and mated queens, and the total histological process took less than 10 h. This method provides the optimization of the histological procedure for research breeding programs that use ovariole number as selection criteria for improving reproduction and production traits.
先前的研究中已描述了计算未交配和已交配的意大利蜜蜂蜂王卵巢管数量的技术。拥有一种系统且快速的方法来获取该测量值有助于加速蜜蜂育种计划，因为可以更快地做出选择决策。这项工作的目的是开发一种高效的组织学方法来保存卵巢，以便在比已发表的方法更短的时间内评估新出现的未交配蜂王以及已交配蜂王的卵巢管数量。所提出的方法产生了适用于计数新出现和已交配蜂王卵巢管的图像，并且整个组织学过程耗时不到10小时。该方法为以卵巢管数量作为改善繁殖和生产性状选择标准的研究育种计划优化了组织学程序。
