Singapore University of Technology & Design, 8 Somapah Road, Singapore 487372, Singapore.
Singapore University of Technology & Design, 8 Somapah Road, Singapore 487372, Singapore; Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore.
Int J Biol Macromol. 2019 Jun 1;130:1009-1017. doi: 10.1016/j.ijbiomac.2019.02.135. Epub 2019 Mar 6.
Herein, we present the synthesis of surface-oxidized cellulose nanofiber (CNF) hydrogel and characterization with various physicochemical analyses and spectroscopic tools as well as its suitability for cellular encapsulation and delivery. The structure-property relationship as shear thinning, thixotropy, creep-recovery and stimuli responsiveness are explored. The CNF hydrogel is capable to inject possessing shear thinning behavior at shear rate (10 s) range in the normal injecting process. In time-dependent thixotropy, the hydrogel showed rapid transform from flowable fluid back to structured hydrogel fully recovering in less than 60 s. The presence of cell-culture media did not alter shear thinning behavior of CNF hydrogel and showed increased thixotropicity with respect to the control gel. The CNF hydrogel forms 3D structures, without any crosslinker, with a wide range of tunable moduli (36-1000 Pa) based on concentration and external stimuli. The biological characteristics of the thixotropic gels are studied for human breast cancer cells and mouse embryonic stem cells and indicated high cell viability, long-term survival, and spherical morphology.
在此，我们展示了表面氧化的纤维素纳米纤维（CNF）水凝胶的合成及其通过各种物理化学分析和光谱工具的表征，以及其用于细胞封装和递送的适用性。研究了结构-性能关系，如剪切稀化、触变性、蠕变恢复和刺激响应。CNF 水凝胶具有可注射性，在正常注射过程中在剪切速率（10s）范围内表现出剪切稀化行为。在时变触变性中，水凝胶在不到 60s 的时间内从可流动的流体迅速转变为完全恢复结构的水凝胶。细胞培养介质的存在并未改变 CNF 水凝胶的剪切稀化行为，并且相对于对照凝胶显示出触变性增加。CNF 水凝胶无需任何交联剂即可形成 3D 结构，其可调模量范围很宽（36-1000Pa），这取决于浓度和外部刺激。研究了触变凝胶的生物学特性，用于人乳腺癌细胞和小鼠胚胎干细胞，结果表明细胞活力高、长期存活和球形形态。
