van Straaten H W, Hekking J W, Drukker J
Acta Histochem Suppl. 1986;32:185-90.
In this paper a histotechnical procedure with respect to the demonstration of acetylcholinesterase in Technovit 7100 (a recently developed GMA) sections is described. From fixation up to embedding several procedural steps were varied and tested, resulting in a suitable procedure. The embedded tissue or sections can be stored for months at 7 degrees C with only a slight reduction of the enzymic activity. The enzyme also appears relatively temperature resistant and independent of section thickness (2-10 microns). The enzymic activity remains constant during incubation. The technique has been applied to our subject of study in order to qualify and quantify several aspects of the development of neuroblasts.
本文描述了一种在Technovit 7100（一种最近开发的GMA）切片中显示乙酰胆碱酯酶的组织技术程序。从固定到包埋，对几个程序步骤进行了改变和测试，从而得到了一个合适的程序。包埋后的组织或切片可在7摄氏度下保存数月，酶活性仅有轻微降低。该酶似乎也具有相对的温度耐受性，且与切片厚度（2 - 10微米）无关。在孵育过程中酶活性保持恒定。该技术已应用于我们的研究课题，以鉴定和量化神经母细胞发育的几个方面。
