Meyer W, Zschemisch N H, Godynicki S
Anatomical Institute, Hannover School of Veterinary Medicine, Bischofsholer Damm 15 (122), 30173 Hannover, Germany.
Pol J Vet Sci. 2003;6(1):17-28.
Based on careful tissue processing, detailed structural analysis, and histochemical as well as cytophotometrical evaluation of the epidermis, the study presents data with respect to changes of tissue integrity during two storing modes (room temperature and 4 degrees C) and various storage times of the porcine auricle. Structural degeneration was first noted in the barrier region of the epidermis from where such changes spread, independent of storage conditions, from small horizontal necrotic islands and continuously with increasing storage time. The histochemical results corroborated these observations, emphasizing, however, that the lower epidermal layers seemed intact for a longer time period than the upper layers. Cytophotometrical evaluation of histochemical stainings showed, with regard to the enzyme succinate dehydrogenase, that oxidative metabolism was negatively affected in the early stages of storage, whereas epidermal lipids (neutral fats, glycolipids) remained relatively stable, even during storage at room temperature. In conclusion, it was obvious that the barrier region is the most sensitive element of the porcine ear epidermis. Taking into consideration that this part of the epidermis is most important for permeation studies, it seems reasonable to avoid any storage of porcine auricles at room temperature, and to use only auricles that have been stored at 4 degrees C for not more than 4 to 6 hours, immediately after delivery from the slaughter-house. In this way better tissue preservation can be achieved, whereby the use of shinkage-free water-soluble plastic embedding would generally improve the histological control of structural integrity, and the application of an easy to handle enzyme histochemical procedure (e.g. succinate dehydrogenase demonstration) to unfixed fresh-frozen sections would help to control basic aspects of tissue functions. The results are discussed in relation to the use of porcine integument as a model in human dermatological research.
基于对猪耳廓表皮进行的精细组织处理、详细结构分析以及组织化学和细胞光度学评估，该研究呈现了关于猪耳廓在两种储存模式（室温与4摄氏度）及不同储存时间下组织完整性变化的数据。结构退化首先在表皮的屏障区域被观察到，且这种变化会从此处扩散，与储存条件无关，先是形成小的水平坏死岛，并随着储存时间的增加持续发展。组织化学结果证实了这些观察结果，不过强调了表皮下层似乎比上层在更长时间内保持完整。对组织化学染色的细胞光度学评估显示，就琥珀酸脱氢酶而言，氧化代谢在储存早期受到负面影响，而表皮脂质（中性脂肪、糖脂）即使在室温储存期间仍相对稳定。总之，很明显屏障区域是猪耳表皮最敏感的部分。考虑到表皮的这一部分对于渗透研究最为重要，避免在室温下储存猪耳廓似乎是合理的，并且仅使用从屠宰场交付后在4摄氏度下储存不超过4至6小时的耳廓。通过这种方式可以实现更好的组织保存，使用无收缩的水溶性塑料包埋通常会改善对结构完整性的组织学控制，并且对未固定的新鲜冷冻切片应用易于操作的酶组织化学程序（例如琥珀酸脱氢酶显示）将有助于控制组织功能的基本方面。结合猪皮肤在人类皮肤病学研究中的模型应用对结果进行了讨论。
