School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, South Korea.
School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, South Korea; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208, USA.
Bioresour Technol. 2019 Jun;282:63-68. doi: 10.1016/j.biortech.2019.02.124. Epub 2019 Mar 1.
This study aimed to investigate the relationship among dynamic membrane (DM) formation, metabolic flux, and microbial community population in dark fermentative hydrogen production. A continuously stirred tank reactor was equipped with an external submerged polyester screen mesh and inoculated with heat-treated anaerobic sludge without immobilization. DM was successfully developed on the polyester mesh and provided high-rate hydrogen production at 60.5 L H/L.d and 2.39 mol H/mol glucose. DM formation was along with tightly bound extracellular polymeric substances. Flux balance analysis revealed that formation of DM altered the metabolic pathways for acetic acid production from homoacetogenesis to hydrogenesis. Bacterial community analysis suggested that Sporolactobacillaceae would contributed to this metabolic pathway shift. Nevertheless, lactic acid was not accumulated and assumed to be consumed by hydrogen producers including Clostridia.
本研究旨在探讨暗发酵制氢过程中动态膜（DM）形成、代谢通量和微生物群落种群之间的关系。采用配备外部浸没式聚酯筛网的连续搅拌槽式反应器，接种未经固定化的热处理厌氧污泥。DM 在聚酯网上成功形成，并以 60.5 L H/L.d 和 2.39 mol H/mol 葡萄糖的高速率生产氢气。DM 的形成伴随着紧密结合的胞外聚合物。通量平衡分析表明，DM 的形成改变了从同型产乙酸作用到产氢作用的乙酸生产代谢途径。细菌群落分析表明，Sporolactobacillaceae 可能有助于这种代谢途径的转变。然而，乳酸没有积累，并被包括梭菌属在内的产氢菌消耗。
