School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
Bioresour Technol. 2022 Jan;344(Pt A):126205. doi: 10.1016/j.biortech.2021.126205. Epub 2021 Oct 26.
This study aimed a high-rate dark fermentative H production from xylose using a dynamic membrane module bioreactor (DMBR) with a 444-μm pore polyester mesh. 20 g xylose/L was fed continuously to the DMBR at different hydraulic retention times (HRTs) from 12 to 3 h at 37 °C. The maximum average H yield (HY) and H production rate (HPR) at 3 h HRT were found to be 1.40 ± 0.07 mol H/mol xylose and 30.26 ± 1.19 L H/L-d, respectively. The short HRT resulted in the maximum suspended biomass concentration (8.92 ± 0.40 g VSS/L) along with significant attached biomass retention (7.88 ± 0.22 g VSS/L). H was produced by both butyric and acetic acid pathways. Low HY was concurrent with lactic acid production. The bacterial population shifted from non-H producers, such as Lactobacillus and Sporolactobacillus spp., to Clostridium sp., when HY increased. Thus, xylose from lignocellulose is a feasible substrate for dark fermentative H production using DMBR.
本研究旨在利用具有 444μm 孔径聚酯网的动态膜生物反应器（DMBR），从木糖中高效生产黑暗发酵氢气。以 37°C 下 12-3h 的不同水力停留时间（HRT），将 20g/L 的木糖连续进料至 DMBR。在 3h 的 HRT 下，发现最大平均氢气产率（HY）和氢气生成速率（HPR）分别为 1.40±0.07mol H/mol 木糖和 30.26±1.19L H/L-d。较短的 HRT 导致最高的悬浮生物量浓度（8.92±0.40g VSS/L），同时显著保留附着生物量（7.88±0.22g VSS/L）。氢气通过丁酸和乙酸途径产生。低 HY 与乳酸生产同时发生。当 HY 增加时，细菌种群从非氢气生产者（如乳酸杆菌和 Sporolactobacillus 属）转变为梭菌属。因此，木质纤维素中的木糖是使用 DMBR 进行黑暗发酵氢气生产的可行底物。
