Section of Molecular Metabolism and Nutrition, Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Division of Neonatology, Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Pediatr Res. 2019 Jun;85(7):1041-1047. doi: 10.1038/s41390-019-0367-y. Epub 2019 Mar 9.
Phototherapy (PT) is the standard treatment of neonatal unconjugated hyperbilirubinemia. Fluorescent tube (FT)-emitted PT light is known to induce oxidative DNA damage in neonates. Nowadays, however, FTs have largely been replaced by light-emitting diodes (LEDs) for delivering PT. Until now, it is unknown whether LED-PT causes oxidative DNA damage. We aim to determine whether LED-PT induces oxidative DNA damage in hyperbilirubinemic rats.
Adult Gunn rats, with genetically unconjugated hyperbilirubinemia, received LED-PT in the clinically relevant doses of 10 or 30 µW/cm/nm. Urine was collected at 0, 24, and 48 h of PT. A group of young Gunn rats received intensive LED-PT of 100 µW/cm/nm for 24 h. Urine was collected every 8 h and analyzed for the levels of oxidative DNA damage marker 8-hydroxy-2'deoxyguanosine (8-OHdG) and creatinine. DNA damage was evaluated by immunohistochemistry (γH2AX) of skin and spleen samples.
LED-PT of 10 and 30 µW/cm/nm did not affect urinary concentrations of 8-OHdG and creatinine or the 8-OHdG/creatinine ratio. Likewise, intensive LED-PT did not affect the 8-OHdG/creatinine ratio or the number of γH2AX-positive cells in the skin or spleen.
Our results show that LED-PT does not induce oxidative DNA damage in hyperbilirubinemic Gunn rats either at clinically relevant or intensive dosages.
光疗（PT）是治疗新生儿未结合高胆红素血症的标准治疗方法。已知荧光灯管（FT）发出的 PT 光会在新生儿体内引起氧化 DNA 损伤。然而，如今 FT 已在很大程度上被发光二极管（LED）取代，用于提供 PT。到目前为止，尚不清楚 LED-PT 是否会引起氧化 DNA 损伤。我们旨在确定 LED-PT 是否会在高胆红素血症大鼠中引起氧化 DNA 损伤。
成年 Gunn 大鼠，具有遗传性未结合高胆红素血症，接受了临床相关剂量的 10 或 30µW/cm/nm 的 LED-PT。在 PT 后 0、24 和 48 小时收集尿液。一组年轻的 Gunn 大鼠接受了 100µW/cm/nm 的高强度 LED-PT，持续 24 小时。每隔 8 小时收集尿液，并分析氧化 DNA 损伤标志物 8-羟基-2'-脱氧鸟苷（8-OHdG）和肌酐的水平。通过皮肤和脾脏样本的免疫组织化学（γH2AX）评估 DNA 损伤。
10 和 30µW/cm/nm 的 LED-PT 并未影响尿中 8-OHdG 和肌酐的浓度或 8-OHdG/肌酐比值。同样，高强度 LED-PT 也未影响皮肤或脾脏中 8-OHdG/肌酐比值或 γH2AX 阳性细胞的数量。
我们的结果表明，无论是在临床相关剂量还是高强度剂量下，LED-PT 都不会在高胆红素血症 Gunn 大鼠中引起氧化 DNA 损伤。
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