Department of Pediatrics, Beatrix Children's Hospital, University Medical Center Groningen, Groningen, The Netherlands
Department of Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands.
Arch Dis Child Fetal Neonatal Ed. 2020 Nov;105(6):628-633. doi: 10.1136/archdischild-2019-317024. Epub 2020 Apr 8.
Phototherapy is used on the majority of preterm infants with unconjugated hyperbilirubinaemia. The use of fluorescent tube phototherapy is known to induce oxidative DNA damage in infants and has largely been replaced by blue light-emitting diode phototherapy (BLP). To date, it is unknown whether BLP also induces oxidative DNA damage in preterm infants.
To determine whether BLP in preterm infants induces oxidative DNA damage as indicated by 8-hydroxy-2'deoxyguanosine (8-OHdG).
Observational cohort study.
Urine samples (n=481) were collected in a cohort of 40 preterm infants (24-32 weeks' gestational age) during the first week after birth. Urine was analysed for the oxidative marker of DNA damage 8-OHdG and for creatinine, and the 8-OHdG/creatinine ratio was calculated. Durations of phototherapy and levels of irradiance were monitored as well as total serum bilirubin concentrations.
BLP did not alter urinary 8-OHdG/creatinine ratios (B=0.2, 95% CI -6.2 to 6.6) at either low (10-30 µW/cm/nm) or high (>30 µW/cm/nm) irradiance: (B=2.3, 95% CI -5.7 to 10.2 and B=-3.0, 95% CI -11.7 to 5.6, respectively). Also, the 8-OHdG/creatinine ratios were independent on phototherapy duration (B=-0.1, 95% CI -0.3 to 0.1).
BLP at irradiances up to 35 µW/cm/nm given to preterm infants ≤32 weeks' gestation does not affect 8-OHdG, an oxidative marker of DNA damage.
光疗被用于治疗大多数未结合胆红素升高的早产儿。已知荧光灯管光疗会导致婴儿的氧化 DNA 损伤，并且已在很大程度上被蓝光发光二极管光疗 (BLP) 取代。迄今为止，尚不清楚 BLP 是否也会导致早产儿的氧化 DNA 损伤。
确定 BLP 是否会像 8-羟基-2'-脱氧鸟苷 (8-OHdG) 所指示的那样导致早产儿的氧化 DNA 损伤。
观察性队列研究。
在出生后第一周内，收集了 40 名 24-32 周龄早产儿（n=481）的尿液样本。对尿液中的氧化 DNA 损伤标志物 8-OHdG 和肌酐进行分析，并计算 8-OHdG/肌酐比值。同时监测光疗时间和辐照度以及总胆红素浓度。
BLP 并未改变低（10-30 µW/cm/nm）或高（>30 µW/cm/nm）辐照度下的尿液 8-OHdG/肌酐比值（B=0.2，95%CI -6.2 至 6.6）：（B=2.3，95%CI -5.7 至 10.2 和 B=-3.0，95%CI -11.7 至 5.6）。此外，8-OHdG/肌酐比值与光疗时间无关（B=-0.1，95%CI -0.3 至 0.1）。
在 32 周龄以下的早产儿中，给予高达 35 µW/cm/nm 的辐照度的 BLP 不会影响 8-OHdG，这是一种氧化 DNA 损伤的标志物。
