蓝光光疗对早产儿的影响：对 DNA 损伤氧化标志物的影响。

Blue LED phototherapy in preterm infants: effects on an oxidative marker of DNA damage.

机构信息

Department of Pediatrics, Beatrix Children's Hospital, University Medical Center Groningen, Groningen, The Netherlands

Department of Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands.

出版信息

Arch Dis Child Fetal Neonatal Ed. 2020 Nov;105(6):628-633. doi: 10.1136/archdischild-2019-317024. Epub 2020 Apr 8.

DOI:10.1136/archdischild-2019-317024

PMID:32269147

Abstract

BACKGROUND

Phototherapy is used on the majority of preterm infants with unconjugated hyperbilirubinaemia. The use of fluorescent tube phototherapy is known to induce oxidative DNA damage in infants and has largely been replaced by blue light-emitting diode phototherapy (BLP). To date, it is unknown whether BLP also induces oxidative DNA damage in preterm infants.

OBJECTIVE

To determine whether BLP in preterm infants induces oxidative DNA damage as indicated by 8-hydroxy-2'deoxyguanosine (8-OHdG).

DESIGN

Observational cohort study.

METHODS

Urine samples (n=481) were collected in a cohort of 40 preterm infants (24-32 weeks' gestational age) during the first week after birth. Urine was analysed for the oxidative marker of DNA damage 8-OHdG and for creatinine, and the 8-OHdG/creatinine ratio was calculated. Durations of phototherapy and levels of irradiance were monitored as well as total serum bilirubin concentrations.

RESULTS

BLP did not alter urinary 8-OHdG/creatinine ratios (B=0.2, 95% CI -6.2 to 6.6) at either low (10-30 µW/cm/nm) or high (>30 µW/cm/nm) irradiance: (B=2.3, 95% CI -5.7 to 10.2 and B=-3.0, 95% CI -11.7 to 5.6, respectively). Also, the 8-OHdG/creatinine ratios were independent on phototherapy duration (B=-0.1, 95% CI -0.3 to 0.1).

CONCLUSIONS

BLP at irradiances up to 35 µW/cm/nm given to preterm infants ≤32 weeks' gestation does not affect 8-OHdG, an oxidative marker of DNA damage.

摘要

背景

光疗被用于治疗大多数未结合胆红素升高的早产儿。已知荧光灯管光疗会导致婴儿的氧化 DNA 损伤，并且已在很大程度上被蓝光发光二极管光疗 (BLP) 取代。迄今为止，尚不清楚 BLP 是否也会导致早产儿的氧化 DNA 损伤。

目的

确定 BLP 是否会像 8-羟基-2'-脱氧鸟苷 (8-OHdG) 所指示的那样导致早产儿的氧化 DNA 损伤。

设计

观察性队列研究。

方法

在出生后第一周内，收集了 40 名 24-32 周龄早产儿（n=481）的尿液样本。对尿液中的氧化 DNA 损伤标志物 8-OHdG 和肌酐进行分析，并计算 8-OHdG/肌酐比值。同时监测光疗时间和辐照度以及总胆红素浓度。

结果

BLP 并未改变低（10-30 µW/cm/nm）或高（>30 µW/cm/nm）辐照度下的尿液 8-OHdG/肌酐比值（B=0.2，95%CI -6.2 至 6.6）：（B=2.3，95%CI -5.7 至 10.2 和 B=-3.0，95%CI -11.7 至 5.6）。此外，8-OHdG/肌酐比值与光疗时间无关（B=-0.1，95%CI -0.3 至 0.1）。