Department of Cosmeceutics, China Medical University, Taichung, 40402, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, 40447, Taiwan; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan.
Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan.
Phytochemistry. 2019 Jun;162:39-46. doi: 10.1016/j.phytochem.2019.02.016. Epub 2019 Mar 7.
Qing Dai (Naturalis Indigo) is a traditional Chinese medicine (TCM) used as a topical agent in moderate psoriasis, targeting interleukin-17 (IL-17). In this study, it was prepared from the aerial parts of Strobilanthes cusia. Three undescribed indole alkaloid derivatives, indigodoles A-C, along with seven known compounds were isolated from this preparation of Qing Dai and their structures were elucidated from spectroscopic data, including NMR, MS, UV, IR, optical rotation, and CD. As well, most compounds were tested against IL-17. Indigodole C and tryptanthrin could significantly inhibit IL-17 production of Th17 cells. In addition, indigodole A and indirubin showed notably anti-IL-17 gene expression in dose-dependent effects without cytotoxicities toward Th17 and Jurkat cells, respectively. Overall, our studies indicate that the aforementioned indole alkaloids could contribute to anti-IL 17 properties of Qing Dai.
青黛（天然靛蓝）是一种传统的中药，用作中度银屑病的局部制剂，针对白细胞介素-17（IL-17）。在这项研究中，它是从大青属植物的地上部分制备的。从青黛制剂中分离出三种未描述的吲哚生物碱衍生物，即靛蓝 A-C，以及七种已知的化合物，并通过包括 NMR、MS、UV、IR、旋光和 CD 在内的光谱数据阐明了它们的结构。此外，大多数化合物都针对 IL-17 进行了测试。靛蓝 C 和色胺酮可显著抑制 Th17 细胞中 IL-17 的产生。此外，靛蓝 A 和靛玉红在不具有细胞毒性的情况下，分别对 Th17 和 Jurkat 细胞显示出显著的抗 IL-17 基因表达作用，呈剂量依赖性。总的来说，我们的研究表明，上述吲哚生物碱可能有助于青黛的抗 IL-17 特性。
