O'Brien E, Esteso M C, Castaño C, Toledano-Díaz A, Bóveda P, Martínez-Fresneda L, López-Sebastián A, Martínez-Nevado E, Guerra R, López Fernández M, Vega R S, Guillamón F G, Santiago-Moreno J
Departamento de Reproducción Animal, SGIT-INIA, Avda. Puerta de Hierro km 5.9, 28040, Madrid, Spain.
Zoo-Aquarium Madrid, 28011, Madrid, Spain.
Theriogenology. 2019 Apr 15;129:160-167. doi: 10.1016/j.theriogenology.2019.02.024. Epub 2019 Mar 1.
This study compares the effectiveness of the ultra-rapid and conventional freezing of sperm from captive bovids, giraffids, cervids, ursids, a cercopithecid, a delphinid and a phascolarctid. The relationship between sperm head dimensions and cryosurvival was also examined. Compared to conventional freezing, the ultra-rapid freezing of epididymal sperm from the dama gazelle, giraffe and brown bear returned higher cryoresistance ratios (CR, the ratio, in percentage, between the value of the variable after thawing/value before thawing) for sperm viability and motility. In the remaining species, the conventional freezing of epididymal sperm returned better CR values. The conventional freezing method also returned better CR values for ejaculated samples from all species. The head dimensions of both fresh epididymal and ejaculated sperm differed widely among species: for epididymal sperm, dolphin sperm heads were the smallest (7.189 ± 0.049 μm) and dama gazelle sperm heads the largest (43.746 ± 0.291 μm), while for ejaculated sperm, giant panda sperm heads were the smallest (15.926 ± 0.150 μm) and mouflon sperm heads the largest (38.258 ± 0.104 μm). However, no significant correlations were detected between the CR for motility, viability, membrane functional integrity or acrosome integrity and the sperm head area, either for epididymal or ejaculated sperm. In conclusion, ultra-rapid freezing is especially recommended for the cryopreservation of dama gazelle, giraffe and brown bear epididymal sperm. Sperm head dimensions appear not to be useful predictors of how well sperm might survive freezing.
本研究比较了对圈养牛科动物、长颈鹿科动物、鹿科动物、熊科动物、猕猴科动物、海豚科动物和树袋熊科动物的精子进行超快速冷冻和传统冷冻的效果。同时还研究了精子头部尺寸与冷冻存活率之间的关系。与传统冷冻相比，对小鹿瞪羚、长颈鹿和棕熊的附睾精子进行超快速冷冻后，精子活力和运动能力的冷冻抗性比率（CR，解冻后变量值与解冻前变量值的百分比）更高。在其余物种中，附睾精子的传统冷冻获得了更好的CR值。传统冷冻方法对所有物种的射精样本也获得了更好的CR值。新鲜附睾精子和射精精子的头部尺寸在不同物种间差异很大：对于附睾精子，海豚精子头部最小（7.189±0.049μm），小鹿瞪羚精子头部最大（43.746±0.291μm）；而对于射精精子，大熊猫精子头部最小（15.926±0.150μm），摩弗伦羊精子头部最大（38.258±0.104μm）。然而，无论是附睾精子还是射精精子，在运动能力、活力、膜功能完整性或顶体完整性的CR与精子头部面积之间均未检测到显著相关性。总之，特别推荐对小鹿瞪羚、长颈鹿和棕熊的附睾精子进行超快速冷冻保存。精子头部尺寸似乎并非精子冷冻存活情况的有效预测指标。
