Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Centre for Protected Cultivation Technology (CPCT), ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
World J Microbiol Biotechnol. 2019 Mar 9;35(3):51. doi: 10.1007/s11274-019-2623-z.
Protected cultivation of vegetables is often hampered by declining nutrient availability in soil due to year-around farming, which in turn, leads to poor quality and yields, causing serious concern. Our study aimed towards evaluating the potential of novel biofilm formulations-Anabaena or Trichoderma as matrices with Azotobacter sp. as Anabaena-Azotobacter (An-Az) and Trichoderma-Azotobacter (Tr-Az) or together as Anabaena-Trichoderma (An-Tr), on the growth, physiological activities, yield, and changes in the profiles of soil microbial communities in two cultivars (cv. DAPC-6 and cv. Kian) of cucumber (Cucumis sativus). Photosynthetic pigments, evaluated as an index of growth showed two-threefold increase, while elicited activity of defense and antioxidant enzymes was stimulated; this facilitated significant improvement in the plants belonging to the inoculated treatments. Microbial biomass carbon and polysaccharides in soil enhanced by two-threefolds in treatments receiving microbial formulations. Available N in soil increased by 50-90% in An-Az and An-Tr biofilm inoculated treatments, while the availability of P and organic C content of soil improved by 40-60%, over control. PCR-DGGE profiles generated revealed signification modulation of cyanobacterial communities and cultivar-specific differences. Significant enhancement in leaf chlorophyll pigments, soil microbiological parameters and nutrient bio-availabilities along with positive correlation among the analysed parameters, and distinct profiles generated by PCR-DGGE analyses illustrated the promise of these novel inoculants for cucumber.
蔬菜的保护地栽培常常受到常年耕作导致土壤养分供应下降的阻碍，这反过来又导致了较差的品质和产量，引起了严重的关注。我们的研究旨在评估新型生物膜配方——鱼腥藻或木霉作为固氮菌的基质的潜力，固氮菌与鱼腥藻形成鱼腥藻-固氮菌（An-Az）和木霉-固氮菌（Tr-Az），或共同形成鱼腥藻-木霉（An-Tr），对两种黄瓜（Cucumis sativus）品种（cv. DAPC-6 和 cv. Kian）的生长、生理活性、产量以及土壤微生物群落的组成变化的影响。作为生长指标评估的光合色素显示出两到三倍的增加，同时诱导防御和抗氧化酶的活性被刺激；这使得接种处理的植物得到了显著改善。接受微生物配方处理的土壤微生物生物量碳和多糖增加了两到三倍。在鱼腥藻和鱼腥藻-木霉生物膜接种处理中，土壤中的有效氮增加了 50-90%，而土壤中有效磷和有机碳含量提高了 40-60%，与对照相比。PCR-DGGE 图谱显示出蓝细菌群落的显著调节和品种特异性差异。叶片叶绿素色素、土壤微生物学参数和养分生物可利用性显著增强，同时分析参数之间存在正相关关系，PCR-DGGE 分析产生的独特图谱说明了这些新型接种剂对黄瓜的应用前景。
