Wei Xuemin, Bai Xuanjiao, Cao Pei, Wang Gang, Han Jianping, Zhang Zheng
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Chin Herb Med. 2022 Nov 24;15(1):45-56. doi: 10.1016/j.chmed.2022.01.008. eCollection 2023 Jan.
Biofertilizers are reliable alternatives to chemical fertilizers due to various advantages. However, the effect of biofertilizers on yield and quality and the possible mechanisms remain little known. Here, an experiment was conducted in field treated with two kinds of biofertilizers including and microalgae.
A field experiment was conducted on of one year old. The biofertilizers were applied at six treatments: (i) control check, CK; (ii) microalgae, VZ; (iii) , TTB; (iv) microalgae + (1:1), VTA; (v) microalgae + (0.5:1), VTB; (vi) microalgae + (1:0.5), VTC. Here, high-throughput sequencing, ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition, heavy metals content and bioactive compounds, respectively.
Compared to CK, root biomass increased by 29.31%-60.39% ( < 0.001). Meanwhile, bioactive compounds were higher than CK after the application of the biofertilizers, peculiarly in TTB and VTB. However, the content of Pb contents in roots significantly reduced by 46.03% and 37.58% respectively in VTC and TTB ( < 0.05). VTA application notably increased the available nitrogen content by 53.03% ( < 0.05), indicating the improvement of soil fertility. Significantly, bacterial and fungal Chao I diversity indices showed an increasing trend with biofertilizer application ( < 0.05), and biofertilizer amendment enriched the rhizosphere soil with beneficial microorganisms that have abilities on promoting plant growth ( and ), adsorbing heavy metal ( and ), controlling plant pathogen (, and ) and promoting the accumulation of metabolites ( and ).
and microalgae biofertilizers improved the quality and biomass of by altering microbial communities in soil.
由于具有多种优势，生物肥料是化肥的可靠替代品。然而，生物肥料对产量和品质的影响以及可能的作用机制仍鲜为人知。在此，在施用了包括[未提及具体名称]和微藻在内的两种生物肥料的田间进行了一项实验。
在一年生[未提及具体作物]上进行田间试验。生物肥料设置六个处理：（i）对照，CK；（ii）微藻，VZ；（iii）[未提及具体名称]，TTB；（iv）微藻 + [未提及具体名称]（1:1），VTA；（v）微藻 + [未提及具体名称]（0.5:1），VTB；（vi）微藻 + [未提及具体名称]（1:0.5），VTC。在此，分别采用高通量测序、电感耦合等离子体质谱和超高效液相色谱系统地表征微生物多样性和结构组成、重金属含量以及生物活性化合物的变化。
与CK相比，根生物量增加了29.31% - 60.39%（P < 0.001）。同时，施用生物肥料后生物活性化合物高于CK，特别是在TTB和VTB中。然而，VTC和TTB中根中铅含量分别显著降低了46.03%和37.58%（P < 0.05）。施用VTA显著提高了有效氮含量53.03%（P < 0.05），表明土壤肥力得到改善。值得注意的是，细菌和真菌的Chao I多样性指数随着生物肥料的施用呈增加趋势（P < 0.05），并且生物肥料改良使根际土壤富集了具有促进植物生长（[未提及具体微生物名称]和[未提及具体微生物名称]）、吸附重金属（[未提及具体微生物名称]和[未提及具体微生物名称]）、控制植物病原体（[未提及具体微生物名称]、[未提及具体微生物名称]和[未提及具体微生物名称]）以及促进代谢物积累（[未提及具体微生物名称]和[未提及具体微生物名称]）能力的有益微生物。
[未提及具体名称]和微藻生物肥料通过改变土壤中的微生物群落提高了[未提及具体作物]的品质和生物量。
