白细胞介素-2、溶链菌制剂及γ-干扰素对刀豆蛋白A体外诱导小鼠非特异性杀伤细胞的影响。
Effects of interleukin-2, OK-432 and interferon-gamma on in vitro induction of nonspecific killer cells by concanavalin A in mice.
作者信息
Mizushima Y, Iwata M, Sato M, Yano S
出版信息
Tohoku J Exp Med. 1986 Jan;148(1):79-85. doi: 10.1620/tjem.148.79.
Human interleukin-2 (IL-2), OK-432 and mouse interferon-gamma (IFN-gamma) were evaluated for their beneficial effects on in vitro induction of nonspecific killer cells by concanavalin A (Con A). Nonspecific killer cells could be induced by culturing C57BL/6 spleen cells for 4 days with Con A. Con A activated killer cells showed direct cell-mediated cytotoxicity (DCMC) and lectin-dependent cell-mediated cytotoxicity (LDCC) against natural killer (NK) sensitive YAC-1 cells, and LDCC against NK-resistant EL-4 cells in a 4 hr-51Cr release assay. Higher DCMC and LDCC reactivity against YAC-1 cells, and higher LDCC reactivity against EL-4 cells were obtained with spleen cells cultured with Con A plus IL-2, or Con A plus OK-432 than with those cultured with Con A alone. No beneficial effect of IFN-gamma and no additive effects of IL-2 plus OK-432, or IL-2 plus IFN-gamma were observed in the induction of nonspecific killer cells by Con A.
对人白细胞介素 -2（IL -2）、溶链菌制剂（OK -432）和小鼠干扰素 -γ（IFN -γ）进行了评估，观察它们对伴刀豆球蛋白A（Con A）体外诱导非特异性杀伤细胞的有益作用。非特异性杀伤细胞可通过用Con A培养C57BL / 6脾细胞4天来诱导。在4小时的51铬释放试验中，Con A激活的杀伤细胞对自然杀伤（NK）敏感的YAC -1细胞表现出直接细胞介导的细胞毒性（DCMC）和凝集素依赖性细胞介导的细胞毒性（LDCC），对NK抗性的EL -4细胞表现出LDCC。与单独用Con A培养的脾细胞相比，用Con A加IL -2或Con A加OK -432培养的脾细胞对YAC -1细胞具有更高的DCMC和LDCC反应性，对EL -4细胞具有更高的LDCC反应性。在Con A诱导非特异性杀伤细胞的过程中，未观察到IFN -γ的有益作用，也未观察到IL -2加OK -432或IL -2加IFN -γ的相加作用。
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