Laboratory of Cell Pathology, Institute of Cytology of the Russian Academy of Sciences, Saint-Petersburg, Russia.
Laboratory of Nanotechnology, Almazov National Medical Research Center, Saint-Petersburg, Russia.
Cytometry A. 2019 Jul;95(7):730-736. doi: 10.1002/cyto.a.23739. Epub 2019 Mar 9.
Ischemic lesions of the heart, including myocardial infarction, are the most common pathologies of human cardiovascular system. Despite all the research and achievements of medicine in this field, the mortality from this disease remains heavy. Therefore, studying of processes occurring in the myocardium in the early and late postinfarction periods remains important. Rat left ventricular cardiomyocyte (CMC) ploidy, hypertrophy, hyperplasia, and ultrastructure were investigated in 2, 6, and 26 weeks after experimental myocardial infarction, caused by permanent ligation of left coronary artery. Cytofluorimetric study of CMC ploidy revealed no difference between normal, sham-operated, and infarcted animals for all the tested stages. However, interference microscopy indicated significant changes in cells size. CMC dry mass of infarcted rats in 2 weeks after surgery was 1.5 times lower than in control and sham operated groups. Electron microscopy analysis of CMC revealed disruption of sarcomere structure. However, in 6 weeks after surgery CMC dry mass was 1.6 times higher than in control. In 26 weeks after myocardial infarction CMC dry mass exceeded control only in peri-infarction zone. Cell counting showed that the number of left ventricular CMC, reduced as a result of myocardial infarction, was not restored during myocardial remodeling. © 2019 International Society for Advancement of Cytometry.
心肌缺血性病变，包括心肌梗死，是人类心血管系统最常见的病理。尽管医学在这一领域的研究和成就很多，但这种疾病的死亡率仍然很高。因此，研究心肌梗死后早期和晚期发生的过程仍然很重要。通过永久性结扎左冠状动脉造成实验性心肌梗死，在 2、6 和 26 周后，研究了大鼠左心室心肌细胞（CMC）的倍性、肥大、增生和超微结构。CMC 倍性的细胞荧光术研究表明，在所有测试阶段，正常、假手术和梗死动物之间没有差异。然而，干涉显微镜表明细胞大小有显著变化。手术后 2 周梗死大鼠的 CMC 干质量比对照组和假手术组低 1.5 倍。对 CMC 的电子显微镜分析显示肌节结构的破坏。然而，手术后 6 周，CMC 干质量比对照组高 1.6 倍。在心肌梗死后 26 周，只有在梗死周边区，CMC 干质量才超过对照组。细胞计数表明，由于心肌梗死而减少的左心室 CMC 数量在心肌重构过程中未恢复。 © 2019 国际细胞分析促进协会。
