Silva Herlon Victor Rodrigues, Nunes Thalles Gothardo Pereira, Ribeiro Leandro Rodrigues, Freitas Luana Azevedo de, de Oliveira Moacir Franco, Assis Neto Antônio Chaves de, Silva Alexandre Rodrigues, Silva Lúcia Daniel Machado da
Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, CE, Brazil.
Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, CE, Brazil.
Anim Reprod Sci. 2019 Apr;203:84-93. doi: 10.1016/j.anireprosci.2019.02.011. Epub 2019 Feb 26.
The jaguar is categorized as "Near Threatened". Conservation strategies, therefore, are needed which include use of reproductive biotechniques. For implementation of biotechnique use, the reproductive characteristics of the species must be understood, which is currently not the case. This study, therefore, aimed to describe the detailed morphology of jaguar sperm, and to evaluate the sperm mitochondrial activity. Five male adults were used. Slides stained with Rose Bengal were used for morphometric and morphological analyses. The length and the width of the sperm head were measured, as well as the length of the middle piece, the tail, and the total length. Scanning and transmission electron microscopy were used for ultrastructural analysis. Mitochondrial function was assessed using the marker 3,3'-diaminobenzidine (DAB). The results are expressed as means ± SEM. The most significant morphological abnormalities observed were head (9 ± 1.7%) and tail defects (12.5 ± 3.3%). The width and length of the head were 3.6 ± 0.03 μm and 4.9 ± 0.02 μm, respectively. The middle piece measured 9.7 ± 0.3 μm, the tail measured 54.5 ± 4.4 μm, and the total length of the sperm was 59.5 ± 0.1 μm. Electron-lucent regions and approximately 54 mitochondrial spirals in the middle piece were identified in the nucleus using electron microscopy. The greatest percentages of cells were classified as DAB I (46.6 ± 4.9%) and DAB II (38 ± 4.4%). The data provide detailed information on the sperm characteristics of jaguars and can support research on germplasm conservation for the species.
美洲虎被归类为“近危”物种。因此，需要采取包括使用生殖生物技术在内的保护策略。为了实施生物技术的应用，必须了解该物种的生殖特征，但目前情况并非如此。因此，本研究旨在描述美洲虎精子的详细形态，并评估精子的线粒体活性。使用了五只成年雄性美洲虎。用孟加拉玫瑰红染色的载玻片用于形态测量和形态分析。测量了精子头部的长度和宽度，以及中段、尾部的长度和总长度。使用扫描电子显微镜和透射电子显微镜进行超微结构分析。使用3,3'-二氨基联苯胺（DAB）标记评估线粒体功能。结果以平均值±标准误表示。观察到的最显著形态异常是头部（9±1.7%）和尾部缺陷（12.5±3.3%）。头部的宽度和长度分别为3.6±0.03μm和4.9±0.02μm。中段长度为9.7±0.3μm，尾部长度为54.5±4.4μm，精子总长度为59.5±0.1μm。在细胞核中，通过电子显微镜鉴定出中段有电子透明区域和约54个线粒体螺旋。最大比例的细胞被归类为DAB I（46.6±4.9%）和DAB II（38±4.4%）。这些数据提供了关于美洲虎精子特征的详细信息，并可为该物种的种质保护研究提供支持。
