Kozumplík J, Martinek J
Vet Med (Praha). 1986 Apr;31(4):227-32.
Laboratory trials were conducted to study the effect of various concentrations of cloprostenol on the motility and morphological changes of the acrosomes of boar spermatozoa. As found, a cloprostenol concentration of 250 ng per ml of semen to 2500 ng per ml of diluted semen has no adverse effect on the motility of spermatozoa and on the morphological changes of their acrosomes. The concentration of 5000 ng of cloprostenol (in the Oestrophan Spofa product) per 1 ml of diluted semen negatively influences the motility of spermatozoa. An insemination dose of 100 ml of diluted sperm treated with 500 ng of cloprostenol was used for the artificial insemination of 152 sows; 166 sows of the same farm inseminated with untreated semen were used as controls. No gilts were included in the trial. Out of the 152 test sows, 113 conceived after the first insemination, i.e. 74.35%, and the average litter size was 10.04 piglets. In the control group, 125 sows delivered their litters, i.e. 75.30% of the total number, the average litter size being 9.96 piglets. Comparing the reproduction parameters of the experimental and control groups it can be said that the treatment of an insemination dose with 500 ng of cloprostenol immediately before insemination had no influence on the pregnancy rate and on the size of litters.
进行了实验室试验，以研究不同浓度的氯前列醇对公猪精子活力和顶体形态变化的影响。结果发现，每毫升精液中氯前列醇浓度为250纳克至每毫升稀释精液中2500纳克，对精子活力及其顶体的形态变化没有不利影响。每1毫升稀释精液中5000纳克氯前列醇（在Oestrophan Spofa产品中）的浓度会对精子活力产生负面影响。用500纳克氯前列醇处理的100毫升稀释精液的输精剂量用于152头母猪的人工授精；同一猪场166头用未处理精液输精的母猪作为对照。试验中未包括后备母猪。在152头试验母猪中，113头在第一次输精后受孕，即受孕率为74.35%，平均产仔数为10.04头仔猪。在对照组中，125头母猪产仔，即占总数的75.30%，平均产仔数为9.96头仔猪。比较实验组和对照组的繁殖参数，可以说在输精前立即用500纳克氯前列醇处理输精剂量对妊娠率和产仔数没有影响。
