Yang Hong, Rehman Mujeeb Ur, Zhang Shaqiu, Yang Jing, Li Yinqi, Gao Jinhai, Gu Lihong, Wang Mingshu, Jia Renyong, Chen Shun, Liu Mafeng, Zhu Dekang, Zhao Xinxin, Wu Ying, Yang Qiao, Huan Juan, Zhang Ling, Liu Yunya, Tian Bin, Pan Leichang, Cheng Xiaoyue, Cheng Anchun
Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, PR China.
Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, PR China.
Acta Trop. 2019 Jun;194:30-35. doi: 10.1016/j.actatropica.2019.03.008. Epub 2019 Mar 7.
The epidemiology and genetic characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) have been widely studied in human and veterinary settings. ESBL-producing E. coli are generally reported in pigs, poultry, and dairy farm animals. Here, we report on the prevalence and genetic characteristics of beta-lactamase producing E. coli isolated from waterfowl birds in Hainan, China. After phenotypic confirmation, genes encoding bla, bla and bla were detected by polymerase chain reaction (PCR). The isolates were assigned to different phylogenetic groups, and multi-locus sequence typing (MLST). Taken as a whole, 289 (92.9%) out of 311 E. coli isolates from waterfowl birds were confirmed as ESBL phenotypes by double-disk synergy testing. Subsequent PCR analysis revealed that bla was the predominant ESBL gene identified in 146 (46.9%) isolates, followed by the combination of bla and bla in 70 (22.5%) isolates. The majority of these positive isolates were assigned to phylogroup B2 (46.2%) followed by phylogroup A (43.6%). In addition, MLST assigned representative ESBL positive isolates (n = 40) to 18 STs, and ST410 (ST23cplx) was the most prevalent population (22.5%). The high prevalence of CTX-M and STs frequently associating with E. coli infections should be of concern as it poses threats to animal and public health. To the best of our knowledge, this is the first comprehensive study to report on the occurrence of ESBL producing E. coli from waterfowl birds in China.
产超广谱β-内酰胺酶（ESBL）的大肠杆菌的流行病学和遗传特征已在人类和兽医领域得到广泛研究。产ESBL的大肠杆菌通常在猪、家禽和奶牛场动物中被报道。在此，我们报告了从中国海南水禽中分离出的产β-内酰胺酶大肠杆菌的流行情况和遗传特征。经过表型确认后，通过聚合酶链反应（PCR）检测编码bla、bla和bla的基因。将分离株分配到不同的系统发育组，并进行多位点序列分型（MLST）。总体而言，通过双盘协同试验，311株水禽大肠杆菌分离株中有289株（92.9%）被确认为ESBL表型。随后的PCR分析显示，bla是在146株（46.9%）分离株中鉴定出的主要ESBL基因，其次是bla和bla组合存在于70株（22.5%）分离株中。这些阳性分离株大多数被分配到B2系统发育组（46.2%），其次是A系统发育组（43.6%）。此外，MLST将代表性的ESBL阳性分离株（n = 40）分配到18个序列型（ST），其中ST410（ST23cplx）是最常见的群体（22.5%）。CTX-M的高流行率以及经常与大肠杆菌感染相关的序列型应引起关注，因为它对动物和公共卫生构成威胁。据我们所知，这是首次全面报道中国水禽中产ESBL大肠杆菌情况的研究。
