Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme-XM, Beliaghata, Kolkata, 700010, India.
Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme-XM, Beliaghata, Kolkata, 700010, India.
Microbes Infect. 2019 Oct-Nov;21(8-9):368-376. doi: 10.1016/j.micinf.2019.02.007. Epub 2019 Mar 7.
Multiple diarrheagenic enteric bacterial infections cause global morbidity and mortality. A combination vaccine is needed to combat different diarrhea-causing organisms. In our present work, we formulated a combination of antigens from three different diarrheagenic Escherichia coli strains and three different Vibrio cholerae strains. We demonstrated that our newly formulated combination immunogen was able to raise species-specific immunogenicity. This formulation also gave protection against different diarrheagenic E. coli strains in the removable intestinal tie-adult rabbit diarrhea model. However, protective efficacy was not found against the V. cholerae El Tor Ogawa Haitian variant, but challenged with V. cholerae El Tor Inaba or O139 showed protection in rabbits. This is the first report of a single formulated nonliving heat-killed combination immunogen from different diarrheagenic E. coli and V. cholerae that could bestow protection against different bacteria in an animal model.
多种致腹泻性肠细菌感染可导致全球发病率和死亡率。需要一种联合疫苗来对抗不同的腹泻病原体。在我们目前的工作中，我们将三种不同的致腹泻性大肠杆菌菌株和三种不同的霍乱弧菌菌株的抗原组合在一起。我们证明，我们新配制的组合免疫原能够引起种特异性免疫原性。这种配方还能在可移动的肠结扎-成人兔腹泻模型中针对不同的致腹泻性大肠杆菌菌株提供保护。然而，对霍乱弧菌 El Tor Ogawa 海地变体没有发现保护效果，但用霍乱弧菌 El Tor Inaba 或 O139 进行挑战时，兔子表现出了保护作用。这是首次报道的一种由不同致腹泻性大肠杆菌和霍乱弧菌组成的单一配方非活菌热灭活组合免疫原，可在动物模型中针对不同细菌提供最佳保护。
