考马斯亮蓝法测定蛋白制剂中低浓度聚山梨酯 80。
Quantitation of low concentrations of polysorbates 80 in protein formulations by Coomassie brilliant blue.
机构信息
Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China; Institue of Immunology, University of Science and Technology of China, Hefei, Anhui, China.
Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China; Institue of Immunology, University of Science and Technology of China, Hefei, Anhui, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
出版信息
Anal Biochem. 2019 May 15;573:67-72. doi: 10.1016/j.ab.2019.03.001. Epub 2019 Mar 7.
Polysorbate 80, as pharmaceutical excipient and virus inactivating agent, is commonly used in the protein pharmaceutical industry. In this study, a method has been developed for the determination of low concentration of Polysorbate 80 in the presence of high concentration proteins (≤100 mg/ml) and excipients. This colorimetric method is based on the interaction of Polysorbate 80 and Coomassie brilliant blue, and suitable for quantitation of Polysorbate 80 in the range of 10-100 μg/ml. Dozens or hundreds of samples can be quantified simultaneously by using microplate. Besides Polysorbate 80, this method can also be used to determine other types of surfactants in protein solutions, such as Polysorbate 20, Triton X-100, NP40, SDS, Benzalkonium chloride/bromide and PEG4000.
聚山梨酯 80 作为药用辅料和病毒灭活剂，在蛋白药物行业中被广泛使用。在本研究中，开发了一种在存在高浓度蛋白质（≤100mg/ml）和辅料的情况下测定低浓度聚山梨酯 80 的方法。该比色法基于聚山梨酯 80 与考马斯亮蓝的相互作用，适用于测定 10-100μg/ml 范围内的聚山梨酯 80。通过使用微孔板可以同时定量数十个或数百个样品。除了聚山梨酯 80 外，该方法还可用于测定蛋白溶液中的其他类型的表面活性剂，如聚山梨酯 20、吐温 X-100、NP40、SDS、苯扎氯铵/溴化物和 PEG4000。
Zotero 插件