聚丙烯酰胺凝胶的考马斯亮蓝染色。
Coomassie-Brilliant Blue staining of polyacrylamide gels.
作者信息
Arndt Claudia, Koristka Stefanie, Feldmann Anja, Bartsch Holger, Bachmann Michael
机构信息
Carl Gustav Carus University TU Dresden, Dresden, Germany.
出版信息
Methods Mol Biol. 2012;869:465-9. doi: 10.1007/978-1-61779-821-4_40.
PMID:22585511
Abstract
Over the past, a series of staining procedures for proteins were published. The most commonly used staining dye for proteins is still Coomassie-Brilliant Blue. The major reason is Coomassie-Brilliant Blue staining is simple, fast, and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water, a series of procedures including colloidal aqueous procedures were described.
摘要
过去，已经发表了一系列蛋白质染色方法。蛋白质最常用的染色染料仍然是考马斯亮蓝。主要原因是考马斯亮蓝染色简单、快速且灵敏。由于考马斯亮蓝几乎不溶于水，因此描述了一系列方法，包括胶体水溶液法。
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