Ou Xiaowen, Chen Peng, Liu Bi-Feng
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology.
Anal Sci. 2019 Jun 10;35(6):609-618. doi: 10.2116/analsci.19R001. Epub 2019 Mar 8.
A microfluidic device as a pivotal research tool in chemistry and life science is now widely recognized. Indeed, microfluidic techniques have made significant advancements in fundamental research, such as the inherent heterogeneity of single-cells studies in cell populations, which would be helpful in understanding cellular molecular mechanisms and clinical diagnosis of major diseases. Single-cell analyses on microdevices have shown great potential for precise fluid control, cell manipulation, and signal output with rapid and high throughput. Moreover, miniaturized devices also have open functions such as integrating with traditional detection methods, for example, optical, electrochemical or mass spectrometry for single-cell analysis. In this review, we summarized recent advances of single-cell analysis based on various microfluidic approaches from different dimensions, such as in vitro, ex vivo, and in vivo analysis of single cells.
微流控装置作为化学和生命科学中的关键研究工具，现已得到广泛认可。事实上，微流控技术在基础研究方面取得了重大进展，例如细胞群体中单细胞研究的内在异质性，这有助于理解细胞分子机制和重大疾病的临床诊断。微器件上的单细胞分析在精确流体控制、细胞操作以及快速高通量信号输出方面显示出巨大潜力。此外，小型化装置还具有开放功能，例如可与传统检测方法集成，如用于单细胞分析的光学、电化学或质谱检测方法。在本综述中，我们从不同维度总结了基于各种微流控方法的单细胞分析的最新进展，如单细胞的体外、离体和体内分析。
