Bioengineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
R&D Laboratory for Innovative Biotherapeutics, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Anal Sci. 2021 Mar 10;37(3):499-503. doi: 10.2116/analsci.20SCP11. Epub 2020 Dec 4.
The quantitatively controlled organellar transfer between living single cells provides a unique experimental platform to analyze the contribution of organellar heterogeneity on cellular phenotypes. We previously developed a microfluidic device which can perform quantitatively controlled mitochondrial transfer between live single cells by promoting strictured cytoplasmic connections between live single cells, but its application to other organelles is unclear. In this study, we investigated the quantitative properties of peroxisome transfer in our microfluidic device. When cells were fused through a 10 or 4 μm long microtunnel by a Sendai virus envelope-based method, a strictured cytoplasmic connection was achieved with a length corresponding to that of the microtunnel, and a subsequent recovery culture disconnected the fused cells. The peroxisome number being transferred through a 10 μm length of the microtunnel was smaller than that of 4 μm. These data suggest that our microfuidic device can perform a quantitative control of peroxisome transfer.
活的单个细胞之间定量控制的细胞器转移为分析细胞器异质性对细胞表型的贡献提供了一个独特的实验平台。我们之前开发了一种微流控装置，通过促进活的单个细胞之间的结构化细胞质连接，可以在活的单个细胞之间进行定量控制的线粒体转移，但它在其他细胞器中的应用尚不清楚。在这项研究中，我们研究了我们的微流控装置中过氧化物酶体转移的定量特性。当细胞通过基于仙台病毒包膜的方法通过 10 或 4 μm 长的微隧道融合时，通过微隧道的长度实现了结构化的细胞质连接，随后的恢复培养会断开融合的细胞。通过 10 μm 长的微隧道转移的过氧化物酶体数量小于 4 μm。这些数据表明，我们的微流控装置可以实现过氧化物酶体转移的定量控制。
