Zhang Xiaotao, Wang Ruoning, Zhang Li, Wei Jianke, Ruan Yibin, Wang Weiwei, Ji Houwei, Liu Jian
Technology Center, China Tobacco Guizhou Industrial Co., Ltd., Guiyang 550009, China.
Minimal Invasive Center, Tumor Hospital, Sun Yat-Sen University, Guangzhou 510060, China.
Int J Anal Chem. 2019 Feb 3;2019:2105839. doi: 10.1155/2019/2105839. eCollection 2019.
A method for simultaneous determination of acetaldehyde, propionaldehyde, acrolein, and crotonaldehyde in gas phase of cigarette mainstream smoke by headspace gas chromatography-mass spectrometry was developed and validated. Gas phase components of mainstream cigarette smoke were extracted with methanol, and then the samples were separated on a DB 624 (60 m, 0.32 mm x 1.8 mm) column, analyzed with headspace gas chromatography-mass spectrometry, and quantified by isotope internal standard. The linearities of acetaldehyde, propionaldehyde, acrolein, and crotonaldehyde were good ( >0.992). The recoveries of acetaldehyde, propionaldehyde, acrolein, and crotonaldehyde were between 78.5% and 115%. The relative standard deviations were less than 10%. The limits of detection and limits of quantitation were 0.014 g/cigarette ~0.12 g/cigarette and 0.045 g/cigarette ~0.38 g/cigarette, respectively. The method had advantage of high sensitivity, it did not require derivatization of 2,4-dinitrophenylhydrazine and avoided a large number of adverse reactions during the process of derivation to improve the accuracy of result, and it was suitable for quantitative analysis of four aldehydes in gas phase of cigarette mainstream smoke.
建立并验证了一种采用顶空气相色谱 - 质谱法同时测定卷烟主流烟气气相中乙醛、丙醛、丙烯醛和巴豆醛的方法。用甲醇萃取卷烟主流烟气的气相成分，然后将样品在DB 624（60 m，0.32 mm×1.8 mm）色谱柱上分离，采用顶空气相色谱 - 质谱法分析，并用同位素内标法定量。乙醛、丙醛、丙烯醛和巴豆醛的线性良好（>0.992）。乙醛、丙醛、丙烯醛和巴豆醛的回收率在78.5%至115%之间。相对标准偏差小于10%。检测限和定量限分别为0.014 μg/支0.12 μg/支和0.045 μg/支0.38 μg/支。该方法具有灵敏度高的优点，无需2,4 - 二硝基苯肼衍生化，避免了衍生过程中大量的不良反应，提高了结果的准确性，适用于卷烟主流烟气气相中四种醛的定量分析。
