Huang Yun, Wang Yigeng, Miao Mingming, Zhao Qihua, Yang Guangyu
School of Chemical Science and Technology of Yunnan University, Kunming 650091, China.
Se Pu. 2007 Mar;25(2):230-3.
Abstract: The determination of major carbonyl compounds in mainstream cigarette smoke by rapid column high performance liquid chromatography was investigated. The cigarette smoke was collected using a Cambridge filter treated with acidic solution of 2, 4-dinitrophenyl-hydrazine. Formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and butyraldehyde were extracted from the Cambridge filter with 50 mL of 2% pyridine acetonitrile solution. The carbonyl compounds in samples were separated on a ZORBAX Stable Bound rapid column (50 mm x 4. 6 mm, 1. 8 microm) in approximately seven minutes and then determined by high performance liquid chromatography with a diode array detector. The average recoveries were in the range of 89. 1% to 99. 2% and the relative standard deviations (RSDs) were generally below 6. 0%. The eight carbonyl compounds in the mainstream smoke of five brands of cigarettes were determined using this method. This method is faster, simpler and consumes less solvent. It is suitable for rapid analysis of carbonyl compounds in mainstream cigarette smoke.
研究了采用快速柱高效液相色谱法测定主流卷烟烟气中的主要羰基化合物。使用经2,4-二硝基苯肼酸性溶液处理的剑桥滤片收集卷烟烟气。用50 mL 2%吡啶乙腈溶液从剑桥滤片中萃取甲醛、乙醛、丙酮、丙烯醛、丙醛、巴豆醛、2-丁酮和丁醛。样品中的羰基化合物在ZORBAX Stable Bound快速柱（50 mm×4.6 mm，1.8 µm）上于约7分钟内分离，然后用配备二极管阵列检测器的高效液相色谱法测定。平均回收率在89.1%至99.2%范围内，相对标准偏差（RSD）一般低于6.0%。采用该方法测定了五个品牌卷烟主流烟气中的八种羰基化合物。该方法更快、更简便且溶剂消耗更少。适用于主流卷烟烟气中羰基化合物的快速分析。
