Xiao Shu-Hui, Li Gong-Xiang, Quan Lingli
Department of Clinical Laboratory Medicine, The People's Hospital of Linyi, Linyi, Shandong 276003, P.R. China.
The First Department of Respiratory of Central Hospital of Zhuzhou, Zhuzhou, Hunan 412000, P.R. China.
Oncol Lett. 2019 Mar;17(3):2607-2614. doi: 10.3892/ol.2019.9886. Epub 2019 Jan 3.
Long non-coding RNAs (lncRNAs) have been investigated in human carcinogenesis. The lncRNA BX357664 has emerged as a novel lncRNA that was initially recognized by a microarray analysis. The present study aimed to identify the expression and functional roles of lncRNA BX357664 in lung cancer. The transcription level of BX357664 was initially revealed to be downregulated in clinical lung cancer tissues and in a series of lung cancer cell lines. Clinical data demonstrated that the high expression of BX357664 was associated with tumor size, distant metastasis and Tumor-Node-Metastasis stage. Following the overexpression of BX357664 in A549 and 95D cells, the potential of cells to form colonies, as well as the proliferation and motility abilities, were revealed to be decreased. Furthermore, the cell cycle was arrested in the G0/G1 phase by BX357664 modulation. Transwell analysis and a wound-healing assay also demonstrated that overexpression of BX357664 in A549 and 95D cells significantly inhibited cell migration and invasion. These data suggested that BX357664 inhibits cell proliferation and metastasis in lung cancer. The results of the present study provided evidence that BX357664 is a novel lncRNA that may aid in the diagnosis and treatment of lung cancer.
长链非编码RNA（lncRNAs）已在人类致癌过程中得到研究。lncRNA BX357664已成为一种新型lncRNA，最初是通过微阵列分析识别出来的。本研究旨在确定lncRNA BX357664在肺癌中的表达及功能作用。最初发现BX357664的转录水平在临床肺癌组织和一系列肺癌细胞系中下调。临床数据表明，BX357664的高表达与肿瘤大小、远处转移及肿瘤-淋巴结-转移分期相关。在A549和95D细胞中过表达BX357664后，发现细胞形成集落的能力以及增殖和运动能力均下降。此外，通过BX357664调节使细胞周期停滞在G0/G1期。Transwell分析和伤口愈合试验还表明，在A549和95D细胞中过表达BX357664可显著抑制细胞迁移和侵袭。这些数据表明BX357664抑制肺癌细胞的增殖和转移。本研究结果提供了证据，证明BX357664是一种新型lncRNA，可能有助于肺癌的诊断和治疗。
