Liu Fengjun, Wang Xinsheng, Liu Haiyan, Wang Yang, Liu Xiaoqian, Hao Xiaochen, Li Hongguang
Department of General Surgery, Shandong University Qilu Hospital, Jinan, Shandong 250012, P.R. China.
Department of General Surgery, Anqiu People's Hospital, Anqiu, Shandong 262100, P.R. China.
Oncol Lett. 2018 Jun;15(6):8237-8244. doi: 10.3892/ol.2018.8435. Epub 2018 Apr 5.
Colorectal cancer represents a great burden for patients worldwide. Long noncoding RNA BX357664 is an RNA that was identified by microarray technique in renal cell carcinoma. The function of BX357664 in solid tumors remains largely unknown. The present study aimed to investigate the expression profile and functional role of BX357664 in human colorectal cancer progression. The transcription levels of BX357664 were initially examined and . An overexpression plasmid was constructed in order to examine the effects of BX357664 overexpression on cell proliferation, apoptosis, migration and invasion. The results demonstrated that BX357664 was significantly downregulated in clinical colorectal cancer tissues and cell lines. Overexpression of BX357664 decreased cell proliferation rates and cell colony formation capacities in HCT116 and HT-29 cells. Following BX357664 overexpression, HCT116 and HT-29 cells exhibited reduced migration and invasion capacities. Would closure was also blunted by >50% following overexpression of BX357664 in HCT-116 and HT-29 cells. In addition, the cell cycle regulators Cyclin B1, CDC25C and Cyclin D1 as well as the mesenchymal marker N-cadherin were downregulated, whereas the epithelial marker E-cadherin was upregulated by BX357664 overexpression. Finally, HCT116 and HT-29 cell apoptosis was induced and activities of caspase-3 and caspase-9 increased significantly following BX357664 overexpression. The present data suggested that BX257664 negatively regulated cell proliferation and metastasis and promoted cell apoptosis in colorectal cancer. These observations provided novel evidence that BX357664 might serve as a tumor suppressor and a potential therapeutic target in the treatment of colorectal cancer in the clinic.
结直肠癌给全球患者带来了巨大负担。长链非编码RNA BX357664是一种通过微阵列技术在肾细胞癌中鉴定出的RNA。BX357664在实体瘤中的功能在很大程度上仍不清楚。本研究旨在探讨BX357664在人结直肠癌进展中的表达谱及功能作用。首先检测了BX357664的转录水平。构建了过表达质粒，以检测BX357664过表达对细胞增殖、凋亡、迁移和侵袭的影响。结果表明，BX357664在临床结直肠癌组织和细胞系中显著下调。BX357664过表达降低了HCT116和HT - 29细胞的增殖率和细胞集落形成能力。BX357664过表达后，HCT116和HT - 29细胞的迁移和侵袭能力降低。在HCT - 116和HT - 29细胞中过表达BX357664后，伤口闭合也减弱了50%以上。此外，细胞周期调节因子细胞周期蛋白B1、细胞周期蛋白依赖性激酶25C和细胞周期蛋白D1以及间充质标志物N - 钙黏蛋白下调，而上皮标志物E - 钙黏蛋白在BX357664过表达后上调。最后，BX357664过表达诱导了HCT116和HT - 29细胞凋亡，且半胱天冬酶 - 3和半胱天冬酶 - 9的活性显著增加。目前的数据表明，BX257664在结直肠癌中负向调节细胞增殖和转移并促进细胞凋亡。这些观察结果提供了新的证据，表明BX357664可能作为一种肿瘤抑制因子，是临床上治疗结直肠癌的潜在治疗靶点。
