Trinh Tuy An, Lee Dahae, Park Seonju, Kim Seung Hyun, Park Jae Gyu, Kim Ji Hwan, Kang Ki Sung
College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 13120, Republic of Korea.
College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Gyeonggi 21983, Republic of Korea.
Oncol Lett. 2019 Mar;17(3):2953-2959. doi: 10.3892/ol.2019.9926. Epub 2019 Jan 14.
L. () is a medicinal plant used for the treatment of inflammatory diseases in East Asian countries. Numerous stilbenes isolated from have been revealed to possess anticancer effects. The aim of the present study was to evaluate the effect of extracts and compounds isolated from on human gastric cancer cell viability and to elucidate their molecular mechanism of action on the apoptosis pathway. The results demonstrated that aloe-emodin and chrysophanol 1-O-β-D-glucopyranoside, isolated from the methanolic extract of dried rhizomes of , exhibited anti-proliferative effects on the human gastric carcinoma cell line AGS, with IC values of 84.66±0.44 and 68.28±0.29 µM, respectively. The percentage of apoptotic cells increased significantly following treatment with each compound at a concentration of 100 µM, compared with that in the non-treated group in the image-based cytometry assay. Western blot analysis revealed that these compounds activated the caspase cascade and inhibited B-cell lymphoma-2, an anti-apoptotic protein.
L.（）是一种在东亚国家用于治疗炎症性疾病的药用植物。从该植物中分离出的众多芪类化合物已被证实具有抗癌作用。本研究的目的是评估从该植物中分离出的提取物和化合物对人胃癌细胞活力的影响，并阐明它们在凋亡途径上的分子作用机制。结果表明，从该植物干燥根茎的甲醇提取物中分离出的芦荟大黄素和大黄酚1 - O-β - D - 吡喃葡萄糖苷对人胃癌细胞系AGS具有抗增殖作用，IC值分别为84.66±0.44和68.28±0.29μM。在基于图像的细胞计数分析中，与未处理组相比，用每种化合物100μM处理后，凋亡细胞的百分比显著增加。蛋白质免疫印迹分析表明，这些化合物激活了半胱天冬酶级联反应并抑制了抗凋亡蛋白B细胞淋巴瘤-2。
