Zhu Qian, Cai Mu-Yan, Weng De-Sheng, Zhao Jing-Jing, Pan Qiu-Zhong, Wang Qi-Jing, Tang Yan, He Jia, Li Min, Xia Jian-Chuan
Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, P. R. China.
Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou 510060, People's Republic of China.
J Cancer. 2019 Jan 29;10(5):1154-1161. doi: 10.7150/jca.29052. eCollection 2019.
: To evaluate the tumour cell PD-L1 (TC-PD-L1) expression patterns in the local microenvironment of clear cell renal cell carcinoma (ccRCC). : 30 fresh primary ccRCC tissues were used to detect the association between TC-PD-L1 and CD8TILs at mRNA level. The incubation experiment was used to confirm the association between extrinsic TC-PD-L1 expression and IFNγ. A cohort of 135 ccRCC patients treated between January 2009 and August 2013 was included for survival analysis. : Our results confirmed that ccRCC cell lines were capable of expressing PD-L1. The incubation experiment demonstrated the positive correlation of TC-PD-L1 expression with interferon-gamma (IFNγ). Additionally, survival analysis was investigated in 135 ccRCC patients and found no independent correlation of TC-PD-L1 expression in multivariate analysis, whereas more distinct prognostic differences were detected when TC-PD-L1-positive ccRCC were sub-classified as with or without CD8 T cell infiltration. : The intrinsic and extrinsic expression patterns are both detected in ccRCC. High positive rate of TC-PD-L1 correlated closely to the strong infiltration of CD8 TILs. TC-PD-L1-positive ccRCC patients with abundant CD8 TILs infiltration confer the high risk of death and disease relapse.
评估透明细胞肾细胞癌（ccRCC）局部微环境中肿瘤细胞程序性死亡受体配体1（TC-PD-L1）的表达模式。使用30例新鲜原发性ccRCC组织在mRNA水平检测TC-PD-L1与CD8肿瘤浸润淋巴细胞（CD8TILs）之间的关联。采用孵育实验证实外源性TC-PD-L1表达与干扰素γ（IFNγ）之间的关联。纳入2009年1月至2013年8月期间接受治疗的135例ccRCC患者队列进行生存分析。我们的结果证实ccRCC细胞系能够表达PD-L1。孵育实验证明TC-PD-L1表达与干扰素γ（IFNγ）呈正相关。此外，对135例ccRCC患者进行生存分析，在多变量分析中发现TC-PD-L1表达无独立相关性，而当TC-PD-L1阳性的ccRCC根据有无CD8 T细胞浸润进行亚分类时，检测到更明显的预后差异。ccRCC中检测到内在和外在表达模式。TC-PD-L1的高阳性率与CD8 TILs的强浸润密切相关。CD8 TILs浸润丰富的TC-PD-L1阳性ccRCC患者具有高死亡风险和疾病复发风险。
