妊娠缺铁和早发型重度子痫前期中转铁蛋白受体的糖基化谱
Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.
作者信息
Gómez-Gutiérrez Alejandra María, Parra-Sosa Beatriz Elena, Bueno-Sánchez Julio Cesar
机构信息
Professor Facultad de Medicina, Departamento de Fisiología y Bioquímica, Grupo Reproducción, Universidad de Antioquia, Carrera 53 # 61-30, Torre 2, Laboratorio 534, Medellín, Colombia.
Grupo de Investigación Alimentación y Nutrición Humana (GIANH-END-UdeA), Universidad de Antioquia, Calle 62 No 52-59, Torre 1, Laboratorio 413, Medellín, Colombia.
出版信息
J Pregnancy. 2019 Feb 3;2019:9514546. doi: 10.1155/2019/9514546. eCollection 2019.
OBJECTIVE
To examine the expression of hypoxia-inducible factor-1 (HIF-1), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.
METHODS
TfR1 and HIF-1 were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.
RESULTS
There was no difference in the expression of TfR1 and HIF-1 between groups. Lectin blot analysis pointed out an overexpression of galactose 1-4 -acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.
CONCLUSION
The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.
目的
检测缺铁性贫血合并先兆子痫（IDAP）及重度子痫前期妇女胎盘组织中缺氧诱导因子-1（HIF-1）、转铁蛋白受体1（TfR1）及TfR1连接的末端单糖的表达情况。
方法
采用蛋白质免疫印迹法检测TfR1和HIF-1。通过特异性凝集素结合对TfR1进行免疫吸附以鉴定末端单糖。
结果
各组间TfR1和HIF-1的表达无差异。凝集素印迹分析表明，重度子痫前期患者中半乳糖1-4 -N-乙酰葡糖胺（Gal-GlcNAc）和甘露糖表达上调。
结论
Gal-GlcNAc增加可能是由于天线结构增多所致，TfR1的甘露糖聚糖可能表明存在错误折叠或不完整的蛋白质。这些发现可能与子痫前期妇女胎盘TfR1低表达有关。
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