Cesare Alessandra De, Parisi Antonio, Lucchi Alex, Capozzi Loredana, Bianco Angela, Pasquali Frederique, Manfreda Gerardo
Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna.
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Sezione di Putignano, Bari, Italy.
Ital J Food Saf. 2019 Feb 11;7(4):7706. doi: 10.4081/ijfs.2018.7706. eCollection 2018 Dec 31.
The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitted to DNA extraction. Total DNA was sequenced in the 16S rRNA and reads analysed with MG-RAST to classify the colonising bacteria up to the genus level and compare each taxonomic group in terms of mean relative frequency of abundance in conventional and antibiotic free carcasses. Firmicutes displayed abundances always higher than 38% but did not show statistically significative differences between conventional and antibiotic free carcasses. On the contrary, Bacteroidetes and Actinobacteria were significantly higher in antibiotic free then conventional carcasses (21.57 10.95%; 19.29 12.05%), whereas Proteobacteria were higher in the latter (33.19 vs 19.52%). The genera significantly higher in antibiotic free than conventional carcasses were (10.07 1.94%), (3.08 0.77%) and s (1.12 0.16%), while was significantly higher in conventional carcasses (1.38 0.26%). Among Firmicutes, the genera significantly higher in conventional carcasses were (1.45 0.11%) and (3.28 0.56%). The higher abundance of Proteobacteria in conventional carcasses might suggest that hygienic conditions in conventional farms are worse than antibiotic free farms. However, from a food safety point of view, was not detected in both kinds of carcasses and the mean relative frequency of abundance was always lower than 0.4%.
本研究的目的是评估来自传统养殖（n = 15）和无抗生素养殖（n = 15）农场的家禽胴体中的微生物群和微生物危害。在制冷隧道末端从每个单独的胴体获取颈部和胸部皮肤的一份样品，并进行DNA提取。对总DNA进行16S rRNA测序，并用MG-RAST分析读数，以将定殖细菌分类到属水平，并比较传统和无抗生素胴体中每个分类组的平均相对丰度频率。厚壁菌门的丰度始终高于38%，但在传统和无抗生素胴体之间未显示出统计学上的显著差异。相反，拟杆菌门和放线菌门在无抗生素胴体中显著高于传统胴体（21.57±10.95%；19.29±12.05%），而变形菌门在后者中更高（33.19%对19.52%）。在无抗生素胴体中显著高于传统胴体的属是（10.07±1.94%）、（3.08±0.77%）和（1.12±0.16%），而在传统胴体中显著更高（1.38±0.26%）。在厚壁菌门中，在传统胴体中显著更高的属是（1.45±0.11%）和（3.28±0.56%）。传统胴体中变形菌门的较高丰度可能表明传统农场的卫生条件比无抗生素农场差。然而，从食品安全的角度来看，在两种胴体中均未检测到，且平均相对丰度频率始终低于0.4%。
