Xia X, Yu J, Zhao X, Yao Y, Zeng L, Ahmed Z, Shen S, Dang R, Lei C
Key laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., No.78, E-jiao Street, Done-E Country, Shandong Province, 252201, China.
Anim Genet. 2019 Jun;50(3):266-270. doi: 10.1111/age.12774. Epub 2019 Mar 10.
To investigate the mtDNA variation and origin of maternal lineages in South American donkeys and to reassess the domestication of donkeys in northeast Africa, we analyzed sequences (489 bp of the D-loop) from 323 domestic donkeys sampled from Peru, Brazil, Ethiopia and Egypt. Altogether, the 323 sequences displayed 53 different haplotypes (45 in Ethiopia, 14 in Egypt, eight in Peru and six in Brazil). Among the four populations, Egyptian donkeys possessed the highest haplotype diversity (0.910 ± 0.032), followed by Brazilian donkeys (0.879 ± 0.060). The Clade I haplotypes dominated in Peruvian donkeys (65%), whereas Clade II haplotypes dominated in Brazilian donkeys (67%). Estimates of F values showed a high genetic differentiation between Peruvian and Brazilian donkey populations (F = 0.4066), which could be explained by the complex introduction history of South American donkeys. Phylogeographic analysis indicates that northeast Africa could be the most probable domestication center for Clade I donkeys. Analysis of molecular variance confirmed a weak genetic structure in domestic donkey populations among four continents (Europe, Asia, Africa and South America).
为了研究南美驴的线粒体DNA变异和母系起源，并重新评估东北非驴的驯化情况，我们分析了从秘鲁、巴西、埃塞俄比亚和埃及采集的323头家养驴的序列（D环的489bp）。总共，这323个序列显示出53种不同的单倍型（埃塞俄比亚有45种，埃及有14种，秘鲁有8种，巴西有6种）。在这四个种群中，埃及驴的单倍型多样性最高（0.910±0.032），其次是巴西驴（0.879±0.060）。单倍型I在秘鲁驴中占主导地位（65%），而单倍型II在巴西驴中占主导地位（67%）。F值估计显示秘鲁和巴西驴种群之间存在高度遗传分化（F = 0.4066），这可以用南美驴复杂的引入历史来解释。系统地理学分析表明，东北非可能是单倍型I驴最有可能的驯化中心。分子方差分析证实了四大洲（欧洲、亚洲、非洲和南美洲）家养驴种群中存在较弱的遗传结构。
