Department of Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham Street (Slot 516), Little Rock, Arkansas 72205, USA.
Chem Commun (Camb). 2019 Apr 11;55(31):4467-4470. doi: 10.1039/c8cc10091h.
We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.
我们通过亲和纯化和定量 LC-MS/MS 鉴定了 29 种 G-四链体结合蛋白。我们证明 DEAD 盒 RNA 解旋酶 Dbp2、Ded1 和 Mss116 优先在体外与 G-四链体核酸结合，并使 RNA 四链体不稳定，这表明这些解旋酶在破坏 RNA 中四链体结构方面具有新的潜在作用。
