通过反相高效液相色谱法分离和纯化单个神经丝蛋白

Separation and purification of individual neurofilament proteins by reverse-phase high-performance liquid chromatography.

作者信息

Hui K S, Hui M, Chiu F C, Banay-Schwartz M, Deguzman T, Sacchi R S, Lajtha A

出版信息

Anal Biochem. 1986 Mar;153(2):230-4. doi: 10.1016/0003-2697(86)90086-2.

Abstract

A reverse-phase HPLC method was developed to separate individual neurofilament proteins (210,000, 160,000 and 70,000 Da) from the glial fibrillary acid protein. It is useful for analytical or preparative methods, with yields higher than 80%. The method represents improvement over previous methods in speed, efficiency, and purity. Combining this HPLC method with the conventional chromatographic method on DEAE-cellulose, highly purified individual neurofilament proteins can be obtained in large scale.

摘要

已开发出一种反相高效液相色谱法，用于从胶质纤维酸性蛋白中分离出单个神经丝蛋白（210,000、160,000和70,000道尔顿）。该方法对分析或制备方法均有用，产率高于80%。该方法在速度、效率和纯度方面比以前的方法有所改进。将这种高效液相色谱法与传统的DEAE-纤维素色谱法相结合，可大规模获得高度纯化的单个神经丝蛋白。