通过反相C-1高效液相色谱法纯化星形蛋白（星形胶质细胞特异性脑蛋白）。

Purification of astroprotein (astrocyte-specific cerebroprotein) by reversed-phase C-1 HPLC.

作者信息

Morimoto K, Hashimoto T, Maeda Y, Hayakawa T, Tagawa K, Mogami H

出版信息

J Neurochem. 1985 Mar;44(3):990-2. doi: 10.1111/j.1471-4159.1985.tb12915.x.

DOI:10.1111/j.1471-4159.1985.tb12915.x

PMID:3919154

Abstract

A method for purification of astroprotein (an astrocyte-specific cerebroprotein) with HPLC is described. A linear gradient from 30 to 70% acetonitrile in 0.1% trifluoroacetic acid (pH 2.2) was applied to the reversed-phase C-1 (particle size 10 micron) column. Cerebroproteins from the crude extract from human glioma were clearly separated by this procedure. Highly purified astroprotein was homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has immunoreactivity to antiserum against astroprotein. Reversed-phase C-1 HPLC offers advantages over previously available preparative techniques in the higher purity and better separation time of the products.

摘要

描述了一种用高效液相色谱法（HPLC）纯化星形蛋白（一种星形胶质细胞特异性脑蛋白）的方法。在反相C-1（粒径10微米）柱上施加0.1%三氟乙酸（pH 2.2）中30%至70%乙腈的线性梯度。通过该程序，人胶质瘤粗提物中的脑蛋白被清晰分离。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，高度纯化的星形蛋白具有均一性，并且对星形蛋白抗血清具有免疫反应性。反相C-1 HPLC相对于先前可用的制备技术具有优势，其产品纯度更高，分离时间更短。