使用二维十二烷基硫酸钠-聚丙烯酰胺凝胶系统，通过化学和酶促消化对异质蛋白质群体进行序列同源性分析。 - Suppr

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Sequence homology analysis of a heterogenous protein population by chemical and enzymic digestion using a two-dimensional sodium dodecyl sulfate-polyacrylamide gel system.

作者信息

Lam K S, Kasper C B

出版信息

Anal Biochem. 1980 Nov 1;108(2):220-6. doi: 10.1016/0003-2697(80)90572-2.

DOI:10.1016/0003-2697(80)90572-2

PMID:6779669

Abstract

摘要

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Sequence homology analysis of a heterogenous protein population by chemical and enzymic digestion using a two-dimensional sodium dodecyl sulfate-polyacrylamide gel system.使用二维十二烷基硫酸钠-聚丙烯酰胺凝胶系统，通过化学和酶促消化对异质蛋白质群体进行序列同源性分析。

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Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.在十二烷基硫酸钠中通过有限蛋白酶解进行肽图谱分析并通过凝胶电泳进行分析。

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Biochemistry. 1975 Mar 25;14(6):1168-77. doi: 10.1021/bi00677a011.

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