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未培养捕食性真菌(捕虫霉目)的新18S rRNA系统发育研究。

A new 18S rRNA phylogeny of uncultured predacious fungi (Zoopagales).

作者信息

Davis William J, Amses Kevin R, James E S, James Timothy Y

机构信息

a Department of Ecology and Evolutionary Biology , The University of Michigan , Ann Arbor , Michigan 48109.

出版信息

Mycologia. 2019 Mar-Apr;111(2):291-298. doi: 10.1080/00275514.2018.1546066. Epub 2019 Mar 11.

Abstract

Previous molecular phylogenetic studies have shown that families in Zoopagales are not monophyletic. To test the monophyly of genera and species in the order, we used a single-cell approach to generate nuclear 18S rRNA (18S) sequences for 10 isolates representing nine taxa. We provide the first sequences for the genus Zoopage and additional sequences for taxa in Cocholonema, Acaulopage, and Zoophagus. Our results reveal that Zoophagus, Zoopage, and Acaulopage tetraceros are not monophyletic. We conclude that morphology alone is not sufficient to delineate genera and species in the order and encourage studies that increase genetic sampling of taxa including type species.

摘要

以往的分子系统发育研究表明,捕虫霉目的科并非单系类群。为了检验该目下属属和种的单系性,我们采用单细胞方法,为代表9个分类单元的10个分离株生成了核18S核糖体RNA(18S)序列。我们提供了捕虫霉属的首个序列,以及科氏虫霉属、无柄虫霉属和噬菌虫霉属分类单元的额外序列。我们的结果显示,噬菌虫霉属、捕虫霉属和四角无柄虫霉并非单系类群。我们得出结论,仅靠形态学不足以界定该目中的属和种,并鼓励开展增加包括模式种在内的分类单元遗传取样的研究。

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本文引用的文献

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Leveraging single-cell genomics to expand the fungal tree of life.利用单细胞基因组学拓展真菌生命之树。
Nat Microbiol. 2018 Dec;3(12):1417-1428. doi: 10.1038/s41564-018-0261-0. Epub 2018 Oct 8.
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Single-cell genome sequencing: current state of the science.单细胞基因组测序:科学现状。
Nat Rev Genet. 2016 Mar;17(3):175-88. doi: 10.1038/nrg.2015.16. Epub 2016 Jan 25.

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