Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, United States.
Department of Plant Pathology, University of Florida, Gainesville, FL, United States.
Mol Phylogenet Evol. 2019 Apr;133:152-163. doi: 10.1016/j.ympev.2019.01.006. Epub 2019 Jan 11.
Previous genome-scale phylogenetic analyses of Fungi have under sampled taxa from Zoopagales; this order contains many predacious or parasitic genera, and most have never been grown in pure culture. We sequenced the genomes of 4 zoopagalean taxa that are predators of amoebae, nematodes, or rotifers and the genome of one taxon that is a parasite of amoebae using single cell sequencing methods with whole genome amplification. Each genome was a metagenome, which was assembled and binned using multiple techniques to identify the target genomes. We inferred phylogenies with both super matrix and coalescent approaches using 192 conserved proteins mined from the target genomes and performed ancestral state reconstructions to determine the ancestral trophic lifestyle of the clade. Our results indicate that Zoopagales is monophyletic. Ancestral state reconstructions provide moderate support for mycoparasitism being the ancestral state of the clade.
先前真菌的全基因组系统发育分析对Zoopagales 目生物的分类群采样不足；该目包含许多捕食性或寄生性的属，其中大多数从未在纯培养中生长过。我们使用单细胞测序方法和全基因组扩增，对 4 种以变形虫、线虫或轮虫为食的Zoopagales 目生物以及一种以变形虫为食的寄生虫的基因组进行了测序。每个基因组都是宏基因组，我们使用多种技术对其进行组装和分类，以确定目标基因组。我们使用从目标基因组中挖掘出的 192 个保守蛋白进行了超级矩阵和共祖方法的系统发育推断，并进行了祖先状态重建，以确定该进化枝的祖先营养方式。我们的结果表明Zoopagales 目是单系的。祖先状态重建为真菌寄生是该进化枝的祖先状态提供了中等程度的支持。
