Department of Biochemistry, Fujita Health University School of Medicine, Toyoake, 470-1192, Japan.
Department of Hygiene, Fujita Health University School of Medicine, Toyoake, 470-1192, Japan.
J Nutr Biochem. 2019 May;67:44-50. doi: 10.1016/j.jnutbio.2019.01.016. Epub 2019 Feb 12.
Global fructose consumption is on the rise; however, maternal high-fructose intake may have adverse effects on offspring. We previously demonstrated that excessive fructose intake by rat dams altered steroidogenic gene transcription in the hippocampus of offspring. Herein, we examined how maternal high-fructose intake influences the regulation of adrenal glucocorticoid levels in offspring. Rat dams received 20% fructose solution during gestation and lactation. After weaning, the offspring were provided normal water. Maternal high-fructose intake did not alter mRNA expression levels of adrenal corticosterone-synthesizing and corticosterone-inactivating proteins or the circulating adrenocorticotropic hormone levels of offspring at postnatal day (PD) 21; however, it increased circulating corticosterone levels and decreased mRNA and protein levels of adrenal 5α-reductase type 1 and 11β-hydroxysteroid dehydrogenase type 2 in offspring at PD160. Furthermore, maternal high-fructose intake enhanced DNA methylation of the adrenal 5α-reductase 1 promoter region in PD160 offspring. Thus, maternal high-fructose intake was found to affect adrenal steroid hormone clearance in adult offspring - at least in part - through epigenetic mechanisms.
全球果糖摄入量呈上升趋势；然而，母体高果糖摄入可能对子代产生不良影响。我们之前的研究表明，母鼠过量摄入果糖会改变子代海马固醇生成基因的转录。在此，我们研究了母体高果糖摄入如何影响子代肾上腺糖皮质激素水平的调节。母鼠在妊娠期和哺乳期接受 20%果糖溶液。断乳后，子代提供正常水。母体高果糖摄入不会改变产后第 21 天（PD）子代肾上腺皮质酮合成和皮质酮失活蛋白的 mRNA 表达水平或循环促肾上腺皮质激素水平；然而，它增加了循环皮质酮水平，并降低了 PD160 子代肾上腺 5α-还原酶 1 型和 11β-羟甾脱氢酶 2 型的 mRNA 和蛋白水平。此外，母体高果糖摄入增强了 PD160 子代肾上腺 5α-还原酶 1 启动子区域的 DNA 甲基化。因此，母体高果糖摄入被发现至少部分通过表观遗传机制影响成年子代的肾上腺类固醇激素清除。
