Cliver Dean O
Food Research Institute (Department of Food Microbiology and Toxicology), W.H.O. Collaborating Centre on Food Virology, and Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706.
J Food Prot. 1981 Nov;44(11):861-865. doi: 10.4315/0362-028X-44.11.861.
This study concerned infections caused by minimal quantities of waterborne enteroviruses. The model system comprised young weanling swine and their homologous enteroviruses; the porcine digestive tract and its enteroviruses are like those of man, and the system affords greater reproducibility and safety than those employing humans or other primates. Subjects swallowed known numbers of viral plaque-forming units (pfu) in 5 ml of drinking water. The body was about 1000 times (600 to 750 for one virus and 1800 to 2500 for another) less likely than a tissue culture to be infected by a given quantity of enterovirus. Doses given after eating, or in four daily portions, produced similar results. No infected animal became ill, despite the reported virulence of the viruses. Chlorination reduced viral infectivity greatly, but short of total extinction, in a single trial. Two newborns were not infected by 20 pfu administered by gavage.
本研究关注由少量水传播肠道病毒引起的感染。模型系统包括幼龄断奶仔猪及其同源肠道病毒；猪的消化道及其肠道病毒与人的相似，该系统比使用人类或其他灵长类动物的系统具有更高的可重复性和安全性。实验对象饮用5毫升含有已知数量病毒空斑形成单位（pfu）的饮用水。相比于组织培养，机体被一定量肠道病毒感染的可能性要低约1000倍（一种病毒为600至750倍，另一种病毒为1800至2500倍）。进食后给药或分四天给药产生了相似的结果。尽管这些病毒有报道称具有毒性，但没有一只受感染的动物发病。在一次试验中，氯化处理极大地降低了病毒的感染性，但并未完全消除。通过灌胃给予20个pfu，两只新生仔猪未被感染。
