Hunter W B, Hiebert E, Webb S E, Tsai J H, Polston J E
University of Florida, Gulf Coast Research and Education Center, 5007 60 St. E., Bradenton 34203-9324.
University of Florida, Plant Pathology Department, 1453 Fifield Hall, Gainesville 32611-0680.
Plant Dis. 1998 Oct;82(10):1147-1151. doi: 10.1094/PDIS.1998.82.10.1147.
The location of tomato mottle virus (ToMoV) and cabbage leaf curl virus (CabLCV) (Geminiviridae, genus Begomovirus) in the whitefly vector Bemisia tabaci B-biotype (Homoptera: Aleyrodidae) was elucidated using a novel technique incorporating indirect immunofluorescent labeling in freshly dissected whiteflies. Begomoviruses were visualized in the whitefly by indirect-fluorescent-microscopy. Polyclonal and monoclonal primary antibodies were used to successfully detect both ToMoV and CabLCV. Both begomoviruses were located in the anterior region of the midgut and filter-chamber of adult whiteflies, with ToMoV detected in the salivary glands. CabLCV was detected at a greater frequency than ToMoV, with a positive detection of 16% (89 out of 560) for CabLCV and 3% (25 out of 840) for ToMoV. Possible sites involved in geminivirus transport from the gut lumen of whiteflies into the hemocoel were located in the filter-chamber and anterior portion of the midgut. The location of these begomoviruses suggests a possible scenario of virus movement through the whitefly, which is discussed.
利用一项在新鲜解剖的烟粉虱中结合间接免疫荧光标记的新技术，阐明了番茄斑驳病毒（ToMoV）和甘蓝叶卷曲病毒（CabLCV）（双生病毒科，菜豆金色花叶病毒属）在烟粉虱B型生物型（同翅目：粉虱科）介体中的定位。通过间接荧光显微镜在烟粉虱中观察到双生病毒。使用多克隆和单克隆一抗成功检测到ToMoV和CabLCV。两种双生病毒均位于成年烟粉虱中肠和滤室的前部区域，在唾液腺中检测到ToMoV。CabLCV的检测频率高于ToMoV，CabLCV的阳性检测率为16%（560个样本中有89个），ToMoV为3%（840个样本中有25个）。双生病毒从烟粉虱肠腔进入血腔的可能位点位于滤室和中肠前部。这些双生病毒的定位表明了病毒在烟粉虱体内移动的一种可能情况，对此进行了讨论。
