Earth and Biological Sciences Directorate, Pacific Northwest National Laboratories, Richland, WA 99354, USA.
Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA.
Int J Mol Sci. 2020 Sep 30;21(19):7241. doi: 10.3390/ijms21197241.
Tomato mottle virus (ToMoV) is a single-stranded DNA (ssDNA) begomovirus transmitted to solanaceous crops by the whitefly species complex (), causing stunted growth, leaf mottling, and reduced yield. Using a genetic repertoire of seven genes, ToMoV pathogenesis includes the manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants ( 'Florida Lanai'), we generated comprehensive protein profiles of leaves subjected to feeding by either viruliferous whiteflies harboring ToMoV, or non-viruliferous whiteflies, or a no-feeding control. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of clip-cage confined whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, tomato's response to ToMoV included proteins associated with translation initiation and elongation as well as plasmodesmata dynamics. In contrast, systemic impacts of ToMoV on younger leaves 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other antiviral plant processes. Our analysis supports previous findings and provides novel insight into tomato's local and systemic response to whitefly feeding and ToMoV infection.
番茄斑萎病毒（ToMoV）是一种单链 DNA（ssDNA）伴生病毒，通过粉虱种复合体（）传播到茄科作物，导致生长迟缓、叶片斑驳和产量降低。利用七个基因的遗传库，ToMoV 发病机制包括操纵多种植物生物过程来规避抗病毒防御。为了进一步了解粉虱取食和粉虱传播的 ToMoV 感染对番茄植株（“佛罗里达拉奈”）的影响，我们对受携带 ToMoV 的毒粉虱、非毒粉虱或无取食对照取食的叶片进行了全面的蛋白质谱分析。通过直接从夹笼限制粉虱取食的部位取样或从取食后 10 天形成的新叶取样，在局部和系统水平上测量了粉虱取食和 ToMoV 感染的影响。在 3 dpf 时，番茄对 ToMoV 的反应包括与翻译起始和延伸以及胞间连丝动态相关的蛋白质。相比之下，10 dpf 时 ToMoV 对较年轻叶片的系统影响更为明显，包括与 mRNA 成熟和输出、RNA 依赖性 DNA 甲基化和其他抗病毒植物过程相关的植物蛋白的特异性变化。我们的分析支持了先前的发现，并为番茄对粉虱取食和 ToMoV 感染的局部和系统反应提供了新的见解。
