Juzwik Jennifer, Cease Kory R, Meyer Jason M
Research Plant Pathologist.
Biological Technician.
Plant Dis. 1998 Feb;82(2):239-243. doi: 10.1094/PDIS.1998.82.2.239.
Field experiments were conducted to determine whether the frequency of Ceratocystis fagacearum and Ophiostoma quercus propagule acquisition by nitidulids visiting oak wilt fungal mats is affected by the presence of O. quercus on the mats. Augmentation sprays with O. quercus were used to achieve different levels of mat colonization by that fungus. The extent of colonization by O. quercus 7 to 14 days after spraying was greatest for postmature mats with open cracks (>0.5 cm) observed on the spray date (P< 0.02). Specifically, all six subsamples assayed per mat for 16 of 18 open, O. quercus-sprayed, postmature mats yielded O. quercus. The frequency of nitidulids with O. quercus was also highest (83%) for those collected from O. quercus-treated, postmature mats that were open on the spray date. The frequency of nitidulids with viable C. fagacearum (88%), however, was also greatest for beetles from the postmature mats. Thus, O. quercus colonization did not affect vector acquisition of C. fagacearum from the mats. In addition, O. quercus vectored by nitidulids probably does not result in natural biological control of overland transmission of C. fagacearum at fresh wound surfaces on healthy oaks, as previously suggested.
进行了田间试验，以确定访问橡树枯萎病真菌菌垫的露尾甲获取板栗疫病菌和栎长喙壳菌繁殖体的频率是否受菌垫上栎长喙壳菌的存在影响。使用栎长喙壳菌进行强化喷雾，以实现该真菌对菌垫的不同定殖水平。喷雾后7至14天，栎长喙壳菌在喷雾当天观察到有开放裂缝（>0.5厘米）的成熟后期菌垫上的定殖程度最大（P<0.02）。具体而言，对18个开放的、喷洒了栎长喙壳菌的成熟后期菌垫中的16个菌垫进行检测，每个菌垫的所有6个亚样本均检测到栎长喙壳菌。从喷雾当天开放的、经栎长喙壳菌处理的成熟后期菌垫上采集的露尾甲携带栎长喙壳菌的频率也最高（83%）。然而，从成熟后期菌垫上采集到的甲虫携带存活的板栗疫病菌的频率（88%）也最高。因此，栎长喙壳菌的定殖并不影响露尾甲从菌垫上获取板栗疫病菌。此外，如先前所述，由露尾甲传播的栎长喙壳菌可能不会对健康橡树新鲜伤口表面的板栗疫病菌陆地传播进行自然生物控制。
