O'Garro Leonard W
Microbial Pathogenicity Research Group, Department of Biological and Chemical Sciences, University of the West Indies, Cave Hill Campus, P.O. Box 64, Bridgetown, Barbados.
Plant Dis. 1998 Aug;82(8):864-870. doi: 10.1094/PDIS.1998.82.8.864.
A total of 5,134 strains of Xanthomonas campestris pv. vesicatoria obtained from diseased pepper and tomato fruits at 22 diverse locations on the East Caribbean islands of Antigua, St. Christopher, St. Lucia, and Grenada were assessed for pathogenicity, taxonomic group phenotypes, and sensitivity to copper, zinc, and streptomycin. Altogether, 15 known races, namely T1, T2, P0T1, P0T2, P1T1, P1T2, P2T1, P2T2, P3T1, P3T2, P4T2, P5T2, P5T3, P6T2, and P6T3, were detected. Races T1, P0T1, P0T2, P1T2, P2T1, and P3T1 were common to all islands except St. Lucia. St. Lucia had races P2T2, P3T2, P4T2, and P6T2 in common with Grenada and two races each from among the four pathotypes in common with Antigua and St. Lucia. Races T1, P1T2, P3T1, and P3T2 were the most abundant strains of the bacterium in Antigua, whereas the two latter pathotypes, as well as races P0T1 and P2T1, were among the most prevalent strains in St. Christopher. In contrast, races P6T2 and P5T2 predominated in St. Lucia, and P6T2, P1T2, and P3T2 were the abundant pathotypes in Grenada. Generally, the largest bacterial spot fruit lesions were associated with the predominant races on each island, and the smallest lesions were generally linked to races capable of overcoming gene Bs2. Only these latter races were similar to group B strains of X. campestris pv. vesicatoria with respect to the presence of β-protein in their cellular protein profiles, ability to hydrolyze starch, and inability to oxidize cis-aconitate. Other race types tested resembled group A strains of the bacterium in their ability to utilize cis-aconitate, inability to hydrolyze starch and pectate, and the presence of the α-protein in profiles of cellular proteins. Copper-resistant strains of X. campestris pv. vesi-catoria constitute 8.7 to 10.6% of the pathogen collections from Antigua and St. Christopher and 44.4 and 66.7% of pathotypes from St. Lucia and Grenada, respectively. Almost all strains were sensitive to zinc. Streptomycin resistance was observed among 62% of the strains in St. Christopher and Grenada and 62.8 to 83.6% of those in Antigua and St. Lucia.
从安提瓜、圣克里斯托弗、圣卢西亚和格林纳达东加勒比群岛22个不同地点的患病辣椒和番茄果实中总共获取了5134株野油菜黄单胞菌辣椒斑点病致病变种，对其致病性、分类群表型以及对铜、锌和链霉素的敏感性进行了评估。总共检测到15个已知小种，即T1、T2、P0T1、P0T2、P1T1、P1T2、P2T1、P2T2、P3T1、P3T2、P4T2、P5T2、P5T3、P6T2和P6T3。除圣卢西亚外，T1、P0T1、P0T2、P1T2、P2T1和P3T1小种在所有岛屿都有。圣卢西亚与格林纳达共有P2T2、P3T2、P4T2和P6T2小种，与安提瓜和圣卢西亚共有四种致病型中的各两个小种。T1、P1T2、P3T1和P3T2小种是安提瓜该细菌中数量最多的菌株，而后两种致病型以及P0T1和P2T1小种是圣克里斯托弗最普遍的菌株。相比之下，P6T2和P5T2小种在圣卢西亚占主导，P6T2、P1T2和P3T2是格林纳达数量多的致病型。一般来说，最大的细菌性斑点果实病斑与每个岛屿上占主导的小种相关，最小的病斑通常与能够克服Bs2基因的小种有关。只有后一类小种在细胞蛋白质谱中β蛋白的存在、水解淀粉的能力以及氧化顺乌头酸的无能方面与野油菜黄单胞菌辣椒斑点病致病变种B组菌株相似。测试的其他小种类型在利用顺乌头酸的能力、水解淀粉和果胶酸盐的无能以及细胞蛋白质谱中α蛋白的存在方面与该细菌的A组菌株相似。野油菜黄单胞菌辣椒斑点病致病变种的耐铜菌株分别占来自安提瓜和圣克里斯托弗病原体样本的8.7%至10.6%，以及来自圣卢西亚和格林纳达致病型的44.4%和66.7%。几乎所有菌株对锌敏感。在圣克里斯托弗和格林纳达62%的菌株以及安提瓜和圣卢西亚62.8%至83.6%的菌株中观察到链霉素抗性。
