Phytopathology. 1999 Apr;89(4):328-35. doi: 10.1094/PHYTO.1999.89.4.328.
ABSTRACT Four hundred thirty-three xanthomonad strains isolated from tomato or pepper plants from 32 different fields in four Caribbean and Central American countries were screened for the ability to hydrolyze starch and sodium polypectate and for resistance to copper and streptomycin. Of these, 95 representative strains were further characterized by various phnetic tests, and 63 of these strains were then analyzed by genomic fingerprinting. Most of the strains (>90%) were tolerant to copper. However, there was much more variability in sensitivity to streptomycin. All strains in Guadeloupe and 93% of the strains in Barbados were sensitive to streptomycin. The majority of strains were typical Xanthomonas campestris pv. vesicatoria group A strains. In Barbados, however, a unique group of strains was identified that was serologically similar to group A strains but was amylolytic. These strains were designated A1. The occurrence of X. campestris pv. vesicatoria group B strains in Central America was found to be limited to two fields in Costa Rica and one in Guatemala. No group B strains were identified in the Caribbean, in contrast to common occurrence in the central United States and in South America. T3 strains were not found in this study, despite the recent increase of such strains in Florida and Mexico. Unique strains from Costa Rica belonging to the X. gardneri group were identified. Little linkage was found among phenotypic and rep-polymerase chain reaction (rep-PCR) genomic fingerprinting profiles of the pathogens except at the species/pathovar level; strains displaying virtually identical fingerprint profiles were found to correspond to distinct races and vice versa. The rep-PCR genomic fingerprinting analyses suggest that certain lineages may have evolved or predominated in specific regions or specific countries.
摘要 从加勒比和中美洲四个国家的 32 个不同田地的番茄或辣椒植物上分离出 433 株黄单胞菌菌株，对它们水解淀粉和聚半乳糖醛酸、以及对铜和链霉素的抗性进行了筛选。其中，95 株有代表性的菌株进一步通过各种生理生化试验进行了特征描述，然后对其中的 63 株菌株进行了基因组指纹图谱分析。大多数菌株（>90%）对铜具有耐受性。然而，对链霉素的敏感性差异很大。瓜德罗普岛的所有菌株和巴巴多斯的 93%的菌株对链霉素敏感。大多数菌株是典型的野油菜黄单胞菌番茄致病变种 A 组菌株。然而，在巴巴多斯，发现了一组独特的菌株，它们在血清学上与 A 组菌株相似，但具有淀粉水解活性。这些菌株被指定为 A1 组。在中美洲，野油菜黄单胞菌番茄致病变种 B 组菌株的发生仅限于哥斯达黎加的两个田块和危地马拉的一个田块。在加勒比地区没有发现 B 组菌株，而在美国中部和南美洲则很常见。尽管佛罗里达州和墨西哥最近增加了此类菌株，但在本研究中未发现 T3 菌株。鉴定出属于 X. gardneri 组的哥斯达黎加特有菌株。除了在种/致病变种水平上，病原体的表型和重复聚合酶链反应（rep-PCR）基因组指纹图谱之间几乎没有关联；发现显示几乎相同指纹图谱的菌株对应于不同的小种，反之亦然。rep-PCR 基因组指纹图谱分析表明，某些谱系可能在特定地区或特定国家进化或占主导地位。
