Block C C, Hill J H, McGee D C
USDA-ARS North Central Regional Plant Introduction Station.
Department of Plant Pathology.
Plant Dis. 1998 Jul;82(7):775-780. doi: 10.1094/PDIS.1998.82.7.775.
Seed transmission of Pantoea stewartii was evaluated by assays of more than 76,000 plants in greenhouse and field grow-out trials. Fourteen P. stewartii-infected seed lots were obtained from two dent corn inbreds and two sweet corn cultivars that were inoculated with either a rifampicin and nalidixic acid-resistant strain (rif-9A) or a wild-type strain (SS104) of P. stewartii. Four additional seed lots were collected from naturally infected inbreds. Percentages of infected kernels ranged from 0.8 to 72%, as determined by agar plating or by individual-kernel enzyme-linked immunosorbent assay (ELISA). Plants grown from this seed were assayed by a stem-printing technique that consisted of cutting and pressing a cross-section of each stem onto agar media. Prints were examined for development of P. stewartii colonies after 24 and 48 h. The transmission rate from seed produced on the inoculated plants was 0.066% (28 of 42,206 plants), based on all seedlings assayed. Transmission was estimated to be 0.14% from infected kernels. The transmission rate from seed produced on naturally infected plants was 0.0029% (1 of 34,924 plants), based on all seedlings, and 0.022% from infected kernels. Seed transmission occurred significantly less often (P = 0.034) from seed produced on naturally infected plants than from seed produced on inoculated plants, probably due to greater kernel damage caused by ear shank inoculation. The rarity of seed transmission of P. stewartii from heavily infected seed lots that would ordinarily be rejected due to poor germination suggests that the likelihood of seed transmission from good quality commercial seed corn is virtually nonexistent.
通过在温室和田间种植试验中对76000多株植物进行测定，评估了斯氏泛菌的种子传播情况。从两个马齿型玉米自交系和两个甜玉米品种中获得了14批感染斯氏泛菌的种子，这些品种接种了对利福平及萘啶酸耐药的菌株（rif - 9A）或斯氏泛菌的野生型菌株（SS104）。另外从自然感染的自交系中收集了4批种子。通过琼脂平板培养法或单粒种子酶联免疫吸附测定（ELISA）确定，感染种子的百分比在0.8%至72%之间。用一种茎印技术对由这些种子长成的植株进行测定，该技术包括将每个茎的横截面切割并压印到琼脂培养基上。在24小时和48小时后检查印模上斯氏泛菌菌落的生长情况。基于所有测定的幼苗，接种植株上产生的种子的传播率为0.066%（42206株植物中有28株）。据估计，感染种子的传播率为0.14%。基于所有幼苗，自然感染植株上产生的种子的传播率为0.0029%（34924株植物中有1株），感染种子的传播率为0.022%。自然感染植株上产生的种子的传播发生率显著低于接种植株上产生的种子（P = 0.034），这可能是由于穗柄接种导致种子损伤更严重。斯氏泛菌从通常因发芽率低而会被拒收的严重感染种子批次中的种子传播情况罕见，这表明优质商业种子玉米发生种子传播的可能性几乎不存在。
