Ota Y, Matsushita N, Nagasawa E, Terashita T, Fukuda K, Suzuki K
Forest Pathologist, Forestry and Forest Products Research Institute, Ibaraki, 305, Japan.
Forest Pathologist, The University of Tokyo.
Plant Dis. 1998 May;82(5):537-543. doi: 10.1094/PDIS.1998.82.5.537.
Pairing tests with isolates of Armillaria in culture were used to identify species of Armillaria and their distribution throughout Japan. The existence of 10 intersterile groups of Armillaria was determined by pairing haploid single spore isolates obtained from 20 basidiocarps from a wide geographic distribution in Japan. Two to four haploid tester isolates from each intersterile group were then paired to biological and morphological species with haploid tester isolates from Europe and North America identified in previous studies. Japanese haploid tester isolates were then paired with 190 haploid or diploid isolates and compatibility reactions were used to identify these to species. Of the 10 intersterile groups identified in Japan, 7 were authenticated as A. gallica, A. nabsnona, A. ostoyae, A. cepistipes, A. mellea, A. sinapina, or A. tabescens. Three of the groups were not compatible with any of the tester species. The distribution and host relationships of the Japanese biological species are also discussed.
采用与培养中的蜜环菌分离株进行配对试验来鉴定蜜环菌的种类及其在日本各地的分布。通过对从日本广泛地理分布的20个担子果中获得的单倍体单孢子分离株进行配对，确定了10个蜜环菌不亲和群。然后将每个不亲和群中的2至4个单倍体测试分离株与先前研究中鉴定的来自欧洲和北美的单倍体测试分离株进行配对，以确定生物学和形态学物种。接着将日本单倍体测试分离株与190个单倍体或二倍体分离株进行配对，并利用亲和反应将它们鉴定到物种。在日本鉴定出的10个不亲和群中，有7个被确认为高卢蜜环菌、纳布氏蜜环菌、奥氏蜜环菌、头状蜜环菌、蜜环菌、芥子蜜环菌或假蜜环菌。其中3个群与任何测试物种都不亲和。还讨论了日本生物学物种的分布及其与寄主的关系。
