Multi-Functional Drug Carrier Micelles With Anti-inflammatory Drug.

The multi-functional micelles poly(-isopropylacrylamide--,-dimethylacrylamide--10 undecanoic acid)/CM-Dextran FeO (PNDU/CM-Dex FeO) were poly (NIPAAm--DMAAm--UA) (PNDU) grafting hydrophilic CM-Dextran FeO which possess pH-dependent temperature response and magnetic response. In this research, anti-inflammation drug Hesperetin was encapsulated by micelles using membrane dialysis method to obtain the different ratio of Hesperetin-embedded PDF, PDF, and PDF. These micelles were characterized by Fourier transform infrared spectroscopy, H-NMR, thermogravimetric analyzer, and superconducting quantum interference device magnetometer. The morphology and particle size of micelles was observed by transmission electron microscopy and dynamic light scattering. The low critical solution temperature of the PDF micelles is in pH 6.6 at about 37.76°C and in pH 7.4 at about 41.70°C. The biocompatibility of micelles was confirmed by cytotoxicity study. Inflammatory inhibition of hesperetin-embedded PDF micelles also studied through RAW264.7. Hesperetin-embed PDF micelles suppressed LPS-induced inflammatory response. Via immunofluorescence cell staining demonstrate that Hesperetin-embed PDF micelles inhibited the activation of NF-κB p60 and markedly attenuated in a drug dose-dependent manner. At a concentration of 1,000 ug/ml, an inflammatory rate can be reduced to 36.9%. Based on these results, the hesperetin-embed PDF micelles had successfully synthesized and enable to carry and release the anti-inflammatory drugs, which instrumental for biomedical therapy and applications.