Immunoelectronmicroscopic localization of actin in ionophore-treated boar sperm.
作者信息
Camatini M, Casale A, Anelli G, Cifarelli M
出版信息
Cell Biol Int Rep. 1986 Apr;10(4):231-8. doi: 10.1016/0309-1651(86)90069-x.
PMID:3085960
Abstract
Actin in ionophore-A23187 treated boar sperm has been localized by indirect immunofluorescence and immunoelectronmicroscopy (IEM), using an anti-actin monoclonal antibody. By IEM, after en bloc staining technique and treatment with 15 nm colloidal gold-IgM complex, actin was found associated with the plasma membrane (PM) and the outer acrosomal membrane vesicles and under the PM of the equatorial segment.
摘要
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